Back to Search
Start Over
Adeno-Associated Virus Harboring Fusion Gene NT4-Ant-Shepherdin Induce Cell Death in Human Lung Cancer Cells
- Source :
- Cancer Investigation. :1-1
- Publication Year :
- 2009
- Publisher :
- Informa UK Limited, 2009.
-
Abstract
- To further enhance anticancer effect of Shepherdin and overcome limitation of peptide therapy, recombinant adeno-associated virus (rAAV) was constructed with following strategies: therapeutic peptide secretory expression and adeno-associated virus gene transfer system. MTT assay and flow cytometric analysis revealed that rAAV harboring fusion gene NT4-Ant-Shepherdin significantly suppressed A549 cell growth in a time-dependent manner and induced apoptosis. In the infected A549 cells, survivin expression level decreased strongly while Caspase-3/7 activities increased significantly. These results indicated that rAAV harboring fusion gene NT4-Ant-Shepherdin may be a novel strategy in lung cancer peptide therapy.
- Subjects :
- Programmed cell death
Cancer Research
Lung Neoplasms
viruses
Recombinant Fusion Proteins
Survivin
Genetic Vectors
Biology
medicine.disease_cause
Virus
Inhibitor of Apoptosis Proteins
Fusion gene
Cell Line, Tumor
medicine
Humans
MTT assay
Nerve Growth Factors
Adeno-associated virus
A549 cell
Cell Death
Gene Transfer Techniques
Genetic Therapy
General Medicine
Dependovirus
Molecular biology
Peptide Fragments
Oncology
Apoptosis
Caspases
Cancer research
Microtubule-Associated Proteins
Subjects
Details
- ISSN :
- 07357907
- Database :
- OpenAIRE
- Journal :
- Cancer Investigation
- Accession number :
- edsair.doi.dedup.....697910f0a043b720fa04fd3df9e5b5d8