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Glyphosate does not substitute for glycine in proteins of actively dividing mammalian cells
- Source :
- BMC Research Notes, Vol 12, Iss 1, Pp 1-7 (2019), BMC Research Notes
- Publication Year :
- 2019
- Publisher :
- BMC, 2019.
-
Abstract
- Objectives Glyphosate (N-phosphonomethyl glycine) and its commercial herbicide formulations have been shown to exert toxicity via various mechanisms. It has been asserted that glyphosate substitutes for glycine in polypeptide chains leading to protein misfolding and toxicity. However, as no direct evidence exists for glycine to glyphosate substitution in proteins, including in mammalian organisms, we tested this claim by conducting a proteomics analysis of MDA-MB-231 human breast cancer cells grown in the presence of 100 mg/L glyphosate for 6 days. Protein extracts from three treated and three untreated cell cultures were analysed as one TMT-6plex labelled sample, to highlight a specific pattern (+/+/+/−/−/−) of reporter intensities for peptides bearing true glyphosate treatment induced-post translational modifications as well as allowing an investigation of the total proteome. Results Comparative statistical analysis of global proteome changes between glyphosate treated and non-treated samples did not show significant differences. Crucially, filtering of data to focus analysis on peptides potentially bearing glycine for glyphosate replacement revealed that the TMT reporter intensity pattern of all candidates showed conclusively that they are all false discoveries, with none displaying the expected TMT pattern for such a substitution. Thus, the assertion that glyphosate substitutes for glycine in protein polypeptide chains is incorrect. Electronic supplementary material The online version of this article (10.1186/s13104-019-4534-3) contains supplementary material, which is available to authorized users.
- Subjects :
- Glycine-tRNA Ligase
Models, Molecular
0301 basic medicine
Glyphosate
Proteome
Direct evidence
Glycine
Gene Expression
lcsh:Medicine
Proteomics
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Cell Line, Tumor
Humans
030212 general & internal medicine
lcsh:Science (General)
lcsh:QH301-705.5
Herbicides
Chemistry
lcsh:R
General Medicine
Neoplasm Proteins
Research Note
030104 developmental biology
Biochemistry
lcsh:Biology (General)
Toxicity
Cancer cell
Protein folding
Protein Processing, Post-Translational
lcsh:Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 17560500
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Research Notes
- Accession number :
- edsair.doi.dedup.....697d62047470ef287646b24fcb02e00b