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Identification and characterization of zebrafish SULT1 ST9, SULT3 ST4, and SULT3 ST5
- Source :
- Aquatic toxicology (Amsterdam, Netherlands).
- Publication Year :
- 2011
-
Abstract
- By searching the GenBank database, we identified sequences encoding three new zebrafish cytosolic sulfotransferases (SULTs). These three new zebrafish SULTs, designated SULT1 ST9, SULT3 ST4, and SULT3 ST5, were cloned, expressed, purified, and characterized. SULT1 ST9 appeared to be mostly involved in the metabolism and detoxification of xenobiotics such as β-naphthol, β-naphthylamine, caffeic acid and gallic acid. SULT3 ST4 showed strong activity toward endogenous compounds such as dehydroepiandrosterone (DHEA), pregnenolone, and 17β-estradiol. SULT3 ST5 showed weaker, but significant, activities toward endogenous compounds such as DHEA and corticosterone, as well as xenobiotics including mestranol, β-naphthylamine, β-naphthol, and butylated hydroxyl anisole (BHA). pH-dependency and kinetic constants of these three enzymes were determined with DHEA, β-naphthol, and 17β-estradiol as substrates. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to examine the expression of these three new zebrafish SULTs at different developmental stages during embryogenesis, through larval development, and on to maturity.
- Subjects :
- Health, Toxicology and Mutagenesis
Molecular Sequence Data
Dehydroepiandrosterone
Aquatic Science
Article
Substrate Specificity
chemistry.chemical_compound
Cytosol
Caffeic acid
medicine
Animals
Cluster Analysis
Gallic acid
Amino Acid Sequence
Zebrafish
chemistry.chemical_classification
biology
Gene Expression Regulation, Developmental
Metabolism
Hydrogen-Ion Concentration
Zebrafish Proteins
biology.organism_classification
Molecular biology
Recombinant Proteins
Kinetics
Enzyme
Biochemistry
chemistry
Pregnenolone
Sulfotransferases
Xenobiotic
Sequence Alignment
medicine.drug
Protein Binding
Subjects
Details
- ISSN :
- 18791514
- Database :
- OpenAIRE
- Journal :
- Aquatic toxicology (Amsterdam, Netherlands)
- Accession number :
- edsair.doi.dedup.....69a25bd00ee500ef345688907dc732b6