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Transcriptome analysis of the biofilm formed by methicillin-susceptible Staphylococcus aureus
- Source :
- Scientific Reports
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- Biofilm formation is regarded as one of the major determinants in the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) as pathogens of medical device-related infection. However, methicillin-susceptible S. aureus (MSSA) can also form biofilm in vitro and such biofilms are resistant to vancomycin. Hence, researching the possible mechanisms of MSSA biofilm formation is urgent and necessary. Here, we used S. aureus ATCC25923 as the model strain and studied gene expression profiles in biofilms after the treatment of ursolic acid and resveratrol using RNA-seq technology. The results showed that only ursolic acid could inhibit biofilm formation, which differed from their applied on the multiple clinical drugs resistant MRSA biofilm. RNA-seq data was validated by examining the expression of six genes involved in biofilm formation by qRT-PCR. These data analysis indicated that the mechanism of the MSSA biofilm formation was different from that of the MRSA, due to absence of accessory gene regulator (agr) function. These findings suggest that biofilms of S. aureus with agr dysfunction may be more resistant than those with agr function. Therefore, the infection from clinical MSSA may be recalcitrant once forming biofilm. Further study is necessary to uncover the mechanisms of biofilm formation in other clinical S. aureus.
- Subjects :
- Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Article
Microbiology
Transcriptome
chemistry.chemical_compound
Ursolic acid
Vancomycin
Stilbenes
medicine
Multidisciplinary
Sequence Analysis, RNA
Gene Expression Profiling
Biofilm
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Methicillin-resistant Staphylococcus aureus
Triterpenes
Anti-Bacterial Agents
Gene expression profiling
RNA, Bacterial
chemistry
Resveratrol
Biofilms
Methicillin Susceptible Staphylococcus Aureus
medicine.drug
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....69a35040191f92b60d63c8e88b57eef7