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Estrogen administration modulates hippocampal GABAergic subpopulations in the hippocampus of trimethyltin-treated rats
- Source :
- Frontiers in Cellular Neuroscience, Vol 9 (2015), Frontiers in Cellular Neuroscience
- Publication Year :
- 2015
- Publisher :
- Frontiers Media S.A., 2015.
-
Abstract
- Given the well-documented involvement of estrogens in the modulation of hippocampal functions in both physiological and pathological conditions, the present study investigates the effects of 17-beta estradiol (E2) administration in the rat model of hippocampal neurodegeneration induced by trimethyltin (TMT) administration (8 mg/kg), characterized by loss of pyramidal neurons in CA1, CA3/hilus hippocampal subfields, associated with astroglial and microglial activation, seizures and cognitive impairment. After TMT/saline treatment, ovariectomized animals received two doses of E2 (0.2 mg/kg intra-peritoneal) or vehicle, and were sacrificed 48 h or 7 days after TMT-treatment. Our results indicate that in TMT-treated animals E2 administration induces the early (48 h) upregulation of genes involved in neuroprotection and synaptogenesis, namely Bcl2, trkB, cadherin 2 and cyclin-dependent-kinase-5. Increased expression levels of glutamic acid decarboxylase (gad) 67, neuropeptide Y (Npy), parvalbumin, Pgc-1α and Sirtuin 1 genes, the latter involved in parvalbumin (PV) synthesis, were also evident. Unbiased stereology performed on rats sacrificed 7 days after TMT treatment showed that although E2 does not significantly influence the extent of TMT-induced neuronal death, significantly enhances the TMT-induced modulation of GABAergic interneuron population size in selected hippocampal subfields. In particular, E2 administration causes, in TMT-treated rats, a significant increase in the number of GAD67-expressing interneurons in CA1 stratum oriens, CA3 pyramidal layer, hilus and dentate gyrus, accompanied by a parallel increase in NPY-expressing cells, essentially in the same regions, and of PV-positive cells in CA1 pyramidal layer. The present results add information concerning the role of in vivo E2 administration on mechanisms involved in cellular plasticity in the adult brain.
- Subjects :
- medicine.medical_specialty
Interneuron
Glutamate decarboxylase
Synaptogenesis
Hippocampus
Biology
Hippocampal formation
Trimethyltin
Neuroprotection
lcsh:RC321-571
Cellular and Molecular Neuroscience
Neuropeptide Y (NPY)
Internal medicine
parvalbumin
medicine
estrogen
hippocampal neurodegeneration
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
Original Research
Settore BIO/16 - ANATOMIA UMANA
Dentate gyrus
glutamic acid decarboxylase
medicine.anatomical_structure
Endocrinology
nervous system
biology.protein
human activities
Parvalbumin
Neuroscience
Subjects
Details
- Language :
- English
- ISSN :
- 16625102
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Frontiers in Cellular Neuroscience
- Accession number :
- edsair.doi.dedup.....69a371fd19e9cef475f36d6f0ab354c7