Supplementary Figure s1 from AQP4 Aggregation State Is a Determinant for Glioma Cell Fate

Supplementary Figure s1. M23-AQP4 C-terminus analysis for the ability to induce morphological changes in glioma cells. a) Schematic representation of the untagged and mCherry-tagged M23-AQP4 isoforms used for the analysis in (b). b) Immuno fluorescence analysis of U87 and U251 cells transfected with tagged and untagged M23-AQP4 isoforms. AQP4 (red) and Phallodin (green) to visualize F-actin. No morphological changes were induced by M23-AQP4 when M23-AQP4 with mCherry tagged C-terminus was used. Scale bar 50 µm. c) Quantification analysis of morphological alterations observed in the experiments described in (a). The histobar shows the percentage of cells with altered morphology. Values are expressed as mean {plus minus} SEM of % of cells with altered cell morphology on the total number of transfected cells per field (**** P