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Structure characterization and anti-leukemia activity of a novel polysaccharide from Angelica sinensis (Oliv.) Diels
- Source :
- International journal of biological macromolecules. 121
- Publication Year :
- 2018
-
Abstract
- A new water-soluble polysaccharide, APS-1II, with a molecular weight of 42.1 kDa was isolated from the roots of Angelica sinensis (Oliv.) Diels. APS-1II consists of arabinose (Ara), glucose (Glc) and fucose (Fuc) with a molar ratio of 2.48:1.05:1.00. The backbone of APS-1II is composed of 1,3-α- l -Araf and 1,6-α- d -Glcp with the branches containing 1,5-α- l -Araf, 1,4-β- d -Glcp, T-β- d -Glcp, 1,3-α- l -Fucp and T-α- l -Fucp. APS-1II inhibited the proliferation of human leukemia K562 and mouse L1210 cells in vitro and markedly prolonged the life span of L1210-bearing mice in vivo. APS-1II also increased the numbers of leukocytes and lymphocytes in peripheral blood, and significantly decreased plasma tumor necrosis factor, interleukin-2 and interferon-γ levels in L1210-bearing mice. Moreover, APS-1II administration concentration-dependently promoted the proliferation of the splenocytes, enhanced phagocytic activity of peritoneal macrophages and cytotoxicity of natural killer cells. These results suggest that APS-1II could effectively inhibit leukemia and induce a protective immune response, and it may be used as a suitable candidate reagent for leukemia therapy.
- Subjects :
- Male
Angelica sinensis
02 engineering and technology
Biochemistry
Fucose
03 medical and health sciences
chemistry.chemical_compound
Mice
Structural Biology
Polysaccharides
medicine
Animals
Humans
Cytotoxicity
Molecular Biology
030304 developmental biology
Cell Proliferation
0303 health sciences
Phagocytes
Leukemia
biology
Hydrolysis
Monosaccharides
General Medicine
021001 nanoscience & nanotechnology
medicine.disease
biology.organism_classification
Molecular biology
Antineoplastic Agents, Phytogenic
Xenograft Model Antitumor Assays
In vitro
Killer Cells, Natural
Molecular Weight
chemistry
L1210 cells
ARAF
0210 nano-technology
K562 Cells
Spleen
K562 cells
Subjects
Details
- ISSN :
- 18790003
- Volume :
- 121
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....69cc349b5e162a8f73929c1ad0ff7f87