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Quantitative proteomics analysis of sporadic parathyroid adenoma tissue samples
- Source :
- Journal of Endocrinological Investigation. 42:577-590
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Molecular pathogenesis of parathyroid tumors is incompletely understood. Identification of novel molecules and understanding their role in parathyroid tumorigenesis by proteomics approach would be informative with potential clinical implications. Adenomatous (n = 5) and normal (n = 2) parathyroid tissue lysates were analyzed for protein profile by LC–MS/MS method and the proteins were classified using bioinformatics tools such as PANTHER and toppfun functional enrichment tool. Identified proteins were further validated by western blotting and qRT-PCR (n = 20). Comparative proteomics analysis revealed that a total of 206 proteins (74 upregulated and 132 downregulated) were differentially expressed (≥ twofold change) in adenomas. Bioinformatics analysis revealed that 48 proteins were associated with plasma membrane, 49 with macromolecular complex, 39 were cytoplasm, 38 were organelle related, 21 were cell junction and 10 were extracellular proteins. These proteins belonged to a diverse protein family such as enzymes, transcription factors, cell signalling, cell adhesion, cytoskeleton proteins, receptors, and calcium-binding proteins. The major biological processes predicted for the proteins were a cellular, metabolic and developmental process, cellular localization, and biological regulation. The differentially expressed proteins were found to be associated with MAPK, phospholipase C (PLC) and phosphatidylinositol (PI) signalling pathways, and with chromatin organization. Western blot and qRT-PCR analysis of three proteins (DNAJC2, ACO2, and PRDX2) validated the LC–MS/MS findings. This exploratory study demonstrates the feasibility of proteomics approach in finding the dysregulated proteins in benign parathyroid adenomas, and our preliminary results suggest that MAPK, PLC and PI signalling pathways and chromatin organization are involved in parathyroid tumorigenesis.
- Subjects :
- Adenoma
Adult
Male
Proteomics
Proteome
Protein family
Endocrinology, Diabetes and Metabolism
Quantitative proteomics
030209 endocrinology & metabolism
Biology
Parathyroid Glands
Young Adult
03 medical and health sciences
0302 clinical medicine
Endocrinology
Western blot
Biomarkers, Tumor
medicine
Humans
Cellular localization
Parathyroid adenoma
medicine.diagnostic_test
Middle Aged
medicine.disease
Cell biology
Blot
Parathyroid Neoplasms
Case-Control Studies
030220 oncology & carcinogenesis
Female
Biological regulation
Follow-Up Studies
Subjects
Details
- ISSN :
- 17208386
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Journal of Endocrinological Investigation
- Accession number :
- edsair.doi.dedup.....6a63a3a80b92abf2c84e8d316ff1b271