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NR4A2 expression is not altered in placentas from cases of growth restriction or preeclampsia, but is reduced in hypoxic cytotrophoblast
- Source :
- Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021), Scientific Reports
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Nuclear Receptor Subfamily 4 Group A Member 2 (NR4A2) transcripts are elevated in the circulation of individuals whose pregnancies are complicated by preterm fetal growth restriction (FGR). In this paper, we show that the cases with preeclampsia (PE) have increased circulating NR4A2 transcripts compared to those with normotensive FGR. We aimed to establish whether the dysfunctional placenta mirrors the increase in NR4A2 transcripts and further, to uncover the function of placental NR4A2. NR4A2 expression was detected in preterm and term placental tissue; expressed higher at term. NR4A2 mRNA expression and protein were not altered in placentas from preterm FGR or PE pregnancies. Hypoxia (1% O2 compared to 8% O2) significantly reduced cytotrophoblast NR4A2 mRNA expression, but not placental explant NR4A2 expression. Silencing cytotrophoblast NR4A2 expression under hypoxia (via short interfering (si)RNAs) did not alter angiogenic Placental Growth Factor, nor anti-angiogenic sFlt-1 mRNA expression or protein secretion, but increased expression of cellular antioxidant, oxidative stress, inflammatory, and growth genes. NR4A2 expression was also not altered in a model of tumour necrosis factor-α-induced endothelial dysfunction, or with pravastatin treatment. Further studies are required to identify the origin of the circulating transcripts in pathological pregnancies, and investigate the function of placental NR4A2.
- Subjects :
- Adult
Male
Placental growth factor
Placenta Diseases
Placenta
Science
Biology
Predictive markers
Antioxidants
Article
Preeclampsia
Andrology
Pre-Eclampsia
Pregnancy
Nuclear Receptor Subfamily 4, Group A, Member 2
Genetics research
medicine
Humans
Gene silencing
RNA, Messenger
Endothelial dysfunction
Hypoxia
Receptor
Placenta Growth Factor
Inflammation
Fetal Growth Retardation
Multidisciplinary
Cytotrophoblast
Intrauterine growth
Hypoxia (medical)
medicine.disease
Trophoblasts
Oxidative Stress
medicine.anatomical_structure
Medicine
Female
Gene expression
medicine.symptom
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....6a7a062b922c455f3992c3481485359a