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Identification of hematopoietic-specific regulatory elements from the CD45 gene and use for lentiviral tracking of transplanted cells
Identification of hematopoietic-specific regulatory elements from the CD45 gene and use for lentiviral tracking of transplanted cells
- Source :
- Experimental hematology. 42(9)
- Publication Year :
- 2014
-
Abstract
- The development of a hematopoietic reporter is crucial for determining the fate of lineages derived from cell-based therapies. A marking system will enable safer embryonic stem and induced pluripotent stem cell-based derivation of blood lineages and facilitate the development of efficient cellular reprogramming strategies based on direct fibroblast conversion. Here we report that the protein tyrosine phosphatase CD45 is an ideal candidate gene on which to base a hematopoietic reporter. CD45 regulatory elements were discovered by analyzing transcription factor chromatin occupancy (ChIP-seq) and promoter nuclease sensitivity (DNase-seq) to identify minimally sufficient sequences required for expression. After cloning the CD45 regulatory elements into an attenuated lentiviral backbone, we found that two transcriptional initiation regions were essential for high-level expression. Expressing CD45 promoters containing these regions and tethered to green fluorescent protein (GFP) in a primary B-cell differentiation assay and a transplantation model resulted in high levels of GFP in lymphoid, myeloid, and nucleated erythroid cells in mouse and human blood cell lineages. Moreover, GFP levels remained high 5 months after secondary transplantation, indicating persistence of the reporter. No CD45-driven GFP expression is observed after fibroblast or embryonic stem cell transduction. The GFP reporter is seen only after embryonic stem cells differentiate into hematopoietic cell progenitors and lineages, suggesting that this hematopoietic reporter system could be useful in validating potential autologous blood cell therapies.
- Subjects :
- Cancer Research
Green Fluorescent Proteins
Biology
Response Elements
Article
Green fluorescent protein
Jurkat Cells
Mice
Genes, Reporter
Transduction, Genetic
Genetics
Animals
Humans
Progenitor cell
Induced pluripotent stem cell
Molecular Biology
Myeloid Progenitor Cells
Lentivirus
Hematopoietic Stem Cell Transplantation
Cell Biology
Hematology
Lymphoid Progenitor Cells
Molecular biology
Embryonic stem cell
Chromatin
Transplantation
Haematopoiesis
Leukocyte Common Antigens
K562 Cells
Reprogramming
Subjects
Details
- ISSN :
- 18732399
- Volume :
- 42
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Experimental hematology
- Accession number :
- edsair.doi.dedup.....6a97b3364f626b4ef6e54d1f6d83cfff