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Beta-eleostearic acid induce apoptosis in T24 human bladder cancer cells through reactive oxygen species (ROS)-mediated pathway
- Source :
- Prostaglandins & Other Lipid Mediators. 99:1-8
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Beta-eleostearic acid (β-ESA, 9E11E13E-18:3), a linolenic acid isomer with a conjugated triene system, is a natural and biologically active compound. Herein, we investigated effects of β-eleostearic acid on T24 human bladder cancer cells. In this study, results showed that β-eleostearic acid had strong cytotoxicity to induce cell apoptosis, which was mediated by reactive oxygen species (ROS) in T24 cells. The cell viability assay results showed that incubation with β-eleostearic acid concentrations of 10-80μmol/L caused a dose- and time-dependent decrease of T24 cell viability, and the IC(50) value was 21.2μmol/L at 24h and 13.1μmol/L at 48h. Annexin V/PI double staining was used to assess apoptosis with flow cytometry. Treatment with β-eleostearic acid caused massive ROS accumulation and GSH decrease, which lead to activation of caspase-3 and down-regulation of Bcl-2 indicating induction of apoptosis. Subsequently, N-acetyl-l-cysteine (NAC) and PEG-catalase effectively blocked the ROS elevated effect of β-eleostearic acid, which suggested that β-eleostearic acid-induced apoptosis involved ROS generated. Additionally, we found that treating T24 cells with β-eleostearic acid induced activation of PPARγ. A PPARγ-activated protein kinase inhibitor was able to partially abrogate the effects of β-eleostearic acid. These results suggested that β-eleostearic acid can induce T24 cells apoptosis via a ROS-mediated pathway which may be involved PPARγ activation.
- Subjects :
- Linolenic Acids
Cell Survival
Physiology
Down-Regulation
Apoptosis
Biology
Biochemistry
Antioxidants
Polyethylene Glycols
Flow cytometry
chemistry.chemical_compound
Annexin
Tumor Cells, Cultured
medicine
Humans
Viability assay
Cytotoxicity
Pharmacology
chemistry.chemical_classification
Reactive oxygen species
medicine.diagnostic_test
Caspase 3
Cell Biology
Glutathione
Catalase
Caspase Inhibitors
Molecular biology
Acetylcysteine
PPAR gamma
Urinary Bladder Neoplasms
chemistry
Cancer cell
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 10988823
- Volume :
- 99
- Database :
- OpenAIRE
- Journal :
- Prostaglandins & Other Lipid Mediators
- Accession number :
- edsair.doi.dedup.....6a9fd42b37da90bbb61dbca971ddc460