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Small RNA profiling analysis of two recombinant strains of potato virus Y in infected tobacco plants
- Source :
- Virus Research. 288:198125
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Plant viral infections lead to accumulation of virus-derived small interfering RNAs (vsiRNAs) as a result of host defense mechanisms. High-throughput sequencing technology enables vsiRNA profiling analyses from virus infected plants, which provide important insights into virus-host interactions. Potato virus Y (PVY) is a detrimental plant pathogen that can infect a variety of solanaceous crops, e.g., potato, tobacco, tomato, and pepper. We analyzed and characterized vsiRNAs derived from Nicotiana tabacum cv. Samsun infected with two recombinant PVY strains, N-Wi and NTN. We observed that the average percentage of vsiRNAs derived from plants infected with N-Wi was higher than from plants infected with NTN, indicating that N-Wi invokes a stronger host response than NTN in tobacco. The size distribution pattern and polarity of vsiRNAs were similar between both virus strains with the 21 and 22 nucleotide (nt) vsiRNA classes as most predominant and the sense/antisense vsiRNAs ratio nearly equal in the 20-24 nt class. However, the percentage of sense vsiRNAs was significantly higher in the 25-26 nt long vsiRNAs. Distinct vsiRNA hotspots, identifying highly abundant reads of different unique vsiRNA sequences, were observed in both viral genomes. Previous studies found an A or U bias at the 5' terminal nucleotide position of 21 nt vsiRNAs; in contrast, our analysis revealed a C and U nucleotide bias. This study provides insights that will help further elucidate differential processing of vsiRNAs in plant antiviral defense.
- Subjects :
- Cancer Research
Small RNA
Nicotiana tabacum
Potyvirus
Defence mechanisms
Genome, Viral
Virus
law.invention
03 medical and health sciences
law
Virology
Tobacco
Sense (molecular biology)
RNA, Small Interfering
Pathogen
Plant Diseases
Solanum tuberosum
030304 developmental biology
0303 health sciences
biology
030306 microbiology
Gene Expression Profiling
fungi
food and beverages
biology.organism_classification
Infectious Diseases
Potato virus Y
Host-Pathogen Interactions
Recombinant DNA
RNA, Viral
Subjects
Details
- ISSN :
- 01681702
- Volume :
- 288
- Database :
- OpenAIRE
- Journal :
- Virus Research
- Accession number :
- edsair.doi.dedup.....6adb75b19afbeffdd593dbf37d36d8c4