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EHD2 regulates adipocyte function and is enriched at cell surface–associated lipid droplets in primary human adipocytes
- Source :
- Molecular Biology of the Cell
- Publication Year :
- 2019
- Publisher :
- American Society for Cell Biology (ASCB), 2019.
-
Abstract
- Adipocytes play a central role in energy balance, and dysfunctional adipose tissue severely affects systemic energy homeostasis. The ATPase EH domain–containing 2 (EHD2) has previously been shown to regulate caveolae, plasma membrane-specific domains that are involved in lipid uptake and signal transduction. Here, we investigated the role of EHD2 in adipocyte function. We demonstrate that EHD2 protein expression is highly up-regulated at the onset of triglyceride accumulation during adipocyte differentiation. Small interfering RNA–mediated EHD2 silencing affected the differentiation process and impaired insulin sensitivity, lipid storage capacity, and lipolysis. Fluorescence imaging revealed localization of EHD2 to caveolae, close to cell surface–associated lipid droplets in primary human adipocytes. These lipid droplets stained positive for glycerol transporter aquaporin 7 and phosphorylated perilipin-1 following adrenergic stimulation. Further, EHD2 overexpression in human adipocytes increased the lipolytic signaling and suppressed the activity of transcription factor PPARγ. Overall, these data suggest that EHD2 plays a key role for adipocyte function.
- Subjects :
- Adult
Male
0301 basic medicine
Cellbiologi
Lipolysis
Cellular differentiation
Caveolin 1
Primary Cell Culture
Adipose tissue
Biology
Caveolae
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
3T3-L1 Cells
Adipocyte
Lipid droplet
Adipocytes
Animals
Humans
Molecular Biology
Transcription factor
Cell Membrane
Membrane Proteins
Cell Differentiation
Lipid Droplets
Articles
Cell Biology
Cell biology
Mice, Inbred C57BL
030104 developmental biology
Adipose Tissue
chemistry
Cell Biology of Disease
Female
Signal transduction
Carrier Proteins
030217 neurology & neurosurgery
Signal Transduction
Subjects
Details
- ISSN :
- 19394586 and 10591524
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Molecular Biology of the Cell
- Accession number :
- edsair.doi.dedup.....6af2a8c96a37913904cd62b39e4ff779