Back to Search
Start Over
A miRNA signature in endothelial cell-derived extracellular vesicles in tumor-bearing mice
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
- Publication Year :
- 2019
- Publisher :
- Nature Publishing Group UK, 2019.
-
Abstract
- Extracellular vesicles (EVs) play important roles in tumor progression by altering immune surveillance, promoting vascular dysfunction, and priming distant sites for organotropic metastases. The miRNA expression patterns in circulating EVs are important diagnostic tools in cancer. However, multiple cell types within the tumor microenvironment (TME) including cancer cells and stromal cells (e.g. immune cells, fibroblasts, and endothelial cells, ECs) contribute to the pool of circulating EVs. Because EVs of different cellular origins have different functional properties, auditing the cargo derived from cell type-specific EVs in the TME is essential. Here, we demonstrate that a murine EC lineage-tracing model (Cdh5-CreERT2:ZSGreenl/s/l mice) can be used to isolate EC-derived extracellular vesicles (EC-EVs). We further show that purified ZSGreen+ EVs express expected EV markers, they are transferable to multiple recipient cells, and circulating EC-EVs from tumor-bearing mice harbor elevated levels of specific miRNAs (e.g. miR-30c, miR-126, miR-146a, and miR-125b) compared to non tumor-bearing counterparts. These results suggest that, in the tumor setting, ECs may systemically direct the function of heterotypic cell types either in the circulation or in different organ micro-environments via the cargo contained within their EVs.
- Subjects :
- Cell type
Stromal cell
Cell
lcsh:Medicine
Breast Neoplasms
Biology
Article
03 medical and health sciences
Extracellular Vesicles
Mice
0302 clinical medicine
Breast cancer
Cell Line, Tumor
medicine
Biomarkers, Tumor
Tumor Microenvironment
Animals
lcsh:Science
030304 developmental biology
0303 health sciences
Tumor microenvironment
Multidisciplinary
Sequence Analysis, RNA
lcsh:R
Epithelial Cells
3. Good health
Cell biology
Up-Regulation
Endothelial stem cell
Gene Expression Regulation, Neoplastic
MicroRNAs
medicine.anatomical_structure
Cell culture
Tumor progression
030220 oncology & carcinogenesis
Cancer cell
lcsh:Q
Female
Tumour angiogenesis
Neoplasm Transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....6b1b81c8dc8217c7537378e62586a88e