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Microconvex Dot-Featured Silk Fibroin Films for Promoting Human Umbilical Vein Endothelial Cell Angiogenesis via Enhancing the Expression of bFGF and VEGF
- Source :
- ACS Biomaterials Science & Engineering. 7:2420-2429
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- Insufficient vascularization of grafts often leads to delayed tissue ingrowth and impaired tissue function in tissue engineering. The surface topography of grafts plays critical roles in angiogenesis. In the present study, we prepared silk fibroin (SF)-based microtopography films with the number of convex dots ranging from 37 to 4835/mm2. The convex dot-featured topography surfaces were characterized by scanning electron microscopy, a Profilm3D optical profilometer, atomic force microscopy, and a contact angle goniometer. The effect of microtopographic films on the proliferation, adhesion, and expression of angiogenic factors of human umbilical vein endothelial cells (HUVECs) was investigated. Our results demonstrated that the SF film surface with 2899 convex dots/mm2 significantly enhanced adhesion, viability, and levels of vascular endothelial growth factors and basic fibroblast growth factors of HUVECs and significantly downregulated the level of α-SMA in human aortic smooth muscle cells, indicating that the microtopographic films could promote angiogenesis. Furthermore, in vitro results showed that HUVEC proliferation was positively correlated with yes-associated protein (YAP) activation, suggesting that the enhanced angiogenesis was mediated via the YAP pathway. Finally, mice subcutaneous embedding model results indicated that the SF film surface with 2899 convex dots/mm2 could significantly enhance angiogenesis in vivo. Altogether, our results showed that the SF film surface with 2899 convex dots/mm2 promoted the angiogenesis of HUVECs and offered a novel angiogenesis-promoting strategy of implant surface design for tissue engineering.
- Subjects :
- Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Angiogenesis
Chemistry
0206 medical engineering
Biomedical Engineering
Fibroin
02 engineering and technology
Adhesion
021001 nanoscience & nanotechnology
Fibroblast growth factor
020601 biomedical engineering
Umbilical vein
Cell biology
Biomaterials
Tissue engineering
In vivo
Human Umbilical Vein Endothelial Cells
Fibroblast Growth Factor 2
Human umbilical vein endothelial cell
Fibroins
0210 nano-technology
Subjects
Details
- ISSN :
- 23739878
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- ACS Biomaterials Science & Engineering
- Accession number :
- edsair.doi.dedup.....6b2dbaab66dd850df3ce533df1c70345