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Social defeat stress causes depression-like behavior with metabolite changes in the prefrontal cortex of rats
- Source :
- PLoS ONE, PLoS ONE, Vol 12, Iss 4, p e0176725 (2017)
- Publication Year :
- 2017
- Publisher :
- Public Library of Science, 2017.
-
Abstract
- Major depressive disorder is a serious mental disorder with high morbidity and mortality. The role of social stress in the development of depression remains unclear. Here, we used the social defeat stress paradigm to induce depression-like behavior in rats, then evaluated the behavior of the rats and measured metabolic changes in the prefrontal cortex using gas chromatography-mass spectrometry. Within the first week after the social defeat procedure, the sucrose preference test (SPT), open field test (OFT), elevated plus maze (EPM) and forced swim test (FST) were conducted to examine the depressive-like and anxiety-like behaviors. For our metabolite analysis, multivariate statistics were applied to observe the distribution of all samples and to differentiate the socially defeated group from the control group. Ingenuity pathway analysis was used to find the potential relationships among the differential metabolites. In the OFT and EPM, there were no significant differences between the two experimental groups. In the SPT and FST, socially defeated rats showed less sucrose intake and longer immobility time compared with control rats. Metabolic profiling identified 25 significant variables with good predictability. Ingenuity pathways analysis revealed that "Hereditary Disorder, Neurological Disease, Lipid Metabolism" was the most significantly altered network. Stress-induced alterations of low molecular weight metabolites were observed in the prefrontal cortex of rats. Particularly, lipid metabolism, amino acid metabolism, and energy metabolism were significantly perturbed. The results of this study suggest that repeated social defeat can lead to metabolic changes and depression-like behavior in rats.
- Subjects :
- 0301 basic medicine
Male
Sucrose
Anhedonia
lcsh:Medicine
Disaccharides
Biochemistry
Open field
Mass Spectrometry
Analytical Chemistry
Social defeat
Rats, Sprague-Dawley
0302 clinical medicine
Spectrum Analysis Techniques
Dietary Sucrose
Metabolites
Medicine and Health Sciences
Medicine
lcsh:Science
Prefrontal cortex
Mammals
Multidisciplinary
Depression
Organic Compounds
Chromatographic Techniques
Brain
Animal Models
Lipids
Chemistry
Experimental Organism Systems
Vertebrates
Physical Sciences
Metabolome
Major depressive disorder
Metabolic Pathways
medicine.symptom
Anatomy
Research Article
Dominance-Subordination
medicine.medical_specialty
Elevated plus maze
Carbohydrates
Prefrontal Cortex
Motor Activity
Research and Analysis Methods
Rodents
Gas Chromatography-Mass Spectrometry
03 medical and health sciences
Model Organisms
Internal medicine
Mental Health and Psychiatry
Animals
Rats, Long-Evans
Social stress
Depressive Disorder
Psychological Tests
business.industry
Mood Disorders
lcsh:R
Organic Chemistry
Organisms
Chemical Compounds
Biology and Life Sciences
Lipid metabolism
medicine.disease
Lipid Metabolism
Rats
Disease Models, Animal
030104 developmental biology
Endocrinology
Metabolism
Amniotes
Multivariate Analysis
Exploratory Behavior
lcsh:Q
business
030217 neurology & neurosurgery
Stress, Psychological
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....6b316cf81848b600b01c5806f51d5349