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Ultraviolet light enhances the bovine serum albumin fixation for acid fast bacilli stain
- Source :
- PLoS ONE, Vol 9, Iss 2, p e89370 (2014), PLoS ONE
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- The use of a liquid culture system such as MGIT broth has greatly improved the sensitivity of isolating mycobacteria in clinical laboratories. Microscopic visualization of acid fast bacilli (AFB) in the culture positive MGIT broth remains the first routine step for rapidly indicating the presence of mycobacteria. We modified an ultraviolet (UV) light fixation process to increase AFB cells adherence to the slide. The retained haze proportion of a 1-cm circle marked area on the smear slide was quantified after the staining procedure indicating the adherence degree of AFB cells. More AFB cells were preserved on the slide after exposure to UV light of either germicidal lamp or UV crosslinker in a time-dependent manner. We demonstrated both the bovine serum albumin (BSA) in MGIT media and UV light exposure were required for enhancing fixation of AFB cells. While applying to AFB stains for 302 AFB positive MGIT broths in clinics, more AFB cells were retained and observed on smear slides prepared by the modified fixation procedure rather than by the conventional method. The modified fixation procedure was thus recommended for improving the sensitivity of microscopic diagnosis of AFB cells in culture positive MGIT broth.
- Subjects :
- Bacterial Diseases
Clinical Pathology
Ultraviolet Rays
Serum albumin
lcsh:Medicine
Stain
Mycobacterium
Microbiology
law.invention
fluids and secretions
Diagnostic Medicine
law
Ultraviolet Radiation
Pathology
Ultraviolet light
Tuberculosis
Bovine serum albumin
Coloring Agents
lcsh:Science
Fixation (histology)
Bacteriological Techniques
Multidisciplinary
Staining and Labeling
biology
Physics
Electromagnetic Radiation
lcsh:R
Serum Albumin, Bovine
Nontuberculous Mycobacteria
Germicidal lamp
bacterial infections and mycoses
Clinical Laboratory Sciences
Staining
respiratory tract diseases
Clinical Microbiology
Infectious Diseases
Gram staining
biology.protein
Medicine
lcsh:Q
Public Health
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....6b44ed86ef98b97747e048b61560ea6c