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SARS-CoV-2 infects an upper airway model derived from induced pluripotent stem cells
- Source :
- STEM CELLS, Stem Cells, 39(10), 1310-1321. AlphaMed Press, Stem Cells (Dayton, Ohio), Djidrovski, I, Georgiou, M, Hughes, G L, Patterson, E I, Casas-Sanchez, A, Pennington, S H, Biagini, G A, Moya-Molina, M, van den Bor, J, Smit, M J, Chung, G, Lako, M & Armstrong, L 2021, ' SARS-CoV-2 infects an upper airway model derived from induced pluripotent stem cells ', Stem Cells, vol. 39, no. 10, pp. 1310-1321 . https://doi.org/10.1002/stem.3422
- Publication Year :
- 2021
-
Abstract
- As one of the primary points of entry of xenobiotic substances and infectious agents into the body, the lungs are subject to a range of dysfunctions and diseases that together account for a significant number of patient deaths. In view of this, there is an outstanding need for in vitro systems in which to assess the impact of both infectious agents and xenobiotic substances of the lungs. To address this issue, we have developed a protocol to generate airway epithelial basal‐like cells from induced pluripotent stem cells, which simplifies the manufacture of cellular models of the human upper airways. Basal‐like cells generated in this study were cultured on transwell inserts to allow formation of a confluent monolayer and then exposed to an air‐liquid interface to induce differentiation into a pseudostratified epithelial construct with a marked similarity to the upper airway epithelium in vivo. These constructs contain the component cell types required of an epithelial model system, produce mucus and functional cilia, and can support SARS‐CoV‐2 infection/replication and the secretion of cytokines in a manner similar to that of in vivo airways. This method offers a readily accessible and highly scalable protocol for the manufacture of upper airway models that could find applications in development of therapies for respiratory viral infections and the assessment of drug toxicity on the human lungs.<br />We have developed a protocol to generate airway epithelial basal‐like cells from induced pluripotent stem cells, which simplifies the manufacture of cellular models of the human upper airways that are capable of supporting SARS‐CoV‐2 infection/replication and the secretion of cytokines in a manner similar to that of in vivo airways.
- Subjects :
- 0301 basic medicine
Cell type
induced pluripotent stem cells
Biology
Virus Replication
Models, Biological
Cell Line
Embryonic Stem Cells/Induced Pluripotent Stem Cells
lung
03 medical and health sciences
0302 clinical medicine
SDG 3 - Good Health and Well-being
In vivo
medicine
Humans
Secretion
Respiratory system
Induced pluripotent stem cell
Lung
SARS-CoV-2
COVID-19
Epithelial Cells
Cell Biology
respiratory system
interleukins (ILs)
Mucus
cytokines
respiratory tract diseases
3. Good health
Cell biology
interleukins
030104 developmental biology
medicine.anatomical_structure
Molecular Medicine
Respiratory epithelium
Inflammation Mediators
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 10665099
- Database :
- OpenAIRE
- Journal :
- STEM CELLS
- Accession number :
- edsair.doi.dedup.....6b6561c13f65bbf01facea5c691ff657
- Full Text :
- https://doi.org/10.1002/stem.3422