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Identification of grade and origin specific cell populations in serous epithelial ovarian cancer by single cell RNA-seq
- Source :
- PLoS ONE, PLoS ONE, Vol 13, Iss 11, p e0206785 (2018)
- Publication Year :
- 2018
- Publisher :
- Public Library of Science (PLoS), 2018.
-
Abstract
- Here we investigated different cell populations within ovarian cancer using single-cell RNA seq: fourteen samples from nine patients with differing grades (high grade, low grade and benign) as well as different origin sites (primary and metastatic tumor site, ovarian in origin and fallopian in origin). We were able to identify sixteen distinct cell populations with specific cells correlated to high grade tumors, low grade tumors, benign and one population unique to a patient with a breast cancer relapse. Furthermore the proportion of these populations changes from primary to metastatic in a shift from mainly epithelial cells to leukocytes with few cancer epithelial cells in the metastases. Differential gene expression shows myeloid lineage cells are the primary cell group expressing soluble factors in primary samples while fibroblasts do so in metastatic samples. The leukocytes that were captured did not seem to be suppressed through known pro-tumor cytokines from any of the cell populations. Single cell RNA-seq is necessary to de-tangle cellular heterogeneity for better understanding of ovarian cancer progression.
- Subjects :
- 0301 basic medicine
B Cells
Colorectal cancer
Cell
lcsh:Medicine
Gene Expression
Epithelium
Metastasis
White Blood Cells
Single-cell analysis
Animal Cells
Medicine and Health Sciences
lcsh:Science
Connective Tissue Cells
Multidisciplinary
Ovarian Cancer
medicine.anatomical_structure
Oncology
Connective Tissue
Cellular Types
Anatomy
Research Article
Stromal cell
Immune Cells
Immunology
Biology
03 medical and health sciences
Breast cancer
Diagnostic Medicine
Genetics
Cancer Detection and Diagnosis
medicine
Antibody-Producing Cells
Blood Cells
lcsh:R
Biology and Life Sciences
Cancers and Neoplasms
Cancer
Epithelial Cells
Cell Biology
Fibroblasts
medicine.disease
Biological Tissue
030104 developmental biology
Metastatic Tumors
Cancer research
lcsh:Q
Stromal Cells
Ovarian cancer
Gynecological Tumors
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....6b6822a3ce47b47bd0f40e76e2c3abb1