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CXCR4‐SF1 bifunctional adipose‐derived stem cells benefit for the treatment of Leydig cell dysfunction‐related diseases
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2020
- Publisher :
- John Wiley and Sons Inc., 2020.
-
Abstract
- Stem cell transplantation is a candidate method for the treatment of Leydig cell dysfunction‐related diseases. However, there are still many problems that limit its clinical application. Here, we report the establishment of CXCR4‐SF1 bifunctional adipose‐derived stem cells (CXCR4‐SF1‐ADSCs) and their reparative effect on Leydig cell dysfunction. CD29+ CD44+ CD34− CD45− ADSCs were isolated from adipose tissue and purified by fluorescence‐activated cell sorting (FACS). Infection with lentiviruses carrying the CXCR4 and SF1 genes was applied to construct CXCR4‐SF1‐ADSCs. The CXCR4‐SF1‐ADSCs exhibited enhanced migration and had the ability to differentiate into Leydig‐like cells in vitro. Furthermore, the bifunctional ADSCs were injected into BPA‐mediated Leydig cell damage model mice via the tail vein. We found that the CXCR4‐SF1‐ADSCs were capable of homing to the injured testes, differentiating into Leydig‐like cells and repairing the deficiency in reproductive function caused by Leydig cell dysfunction. Moreover, we investigated the mechanism underlying SF1‐mediated differentiation and testosterone synthesis in Leydig cells, and the B‐box and SPRY Domain Containing Protein (BSPRY) gene was proposed to be involved in this process. This study provides insight into the treatment of Leydig cell dysfunction‐related diseases.
- Subjects :
- 0301 basic medicine
Male
endocrine system
Receptors, CXCR4
CD34
Adipose tissue
Steroidogenic Factor 1
CXCR4
03 medical and health sciences
adipose‐derived stem cell
Mice
0302 clinical medicine
Testis
medicine
Adipocytes
Animals
Cell Lineage
Cells, Cultured
biology
Leydig cell
urogenital system
CD44
Leydig Cells
Proteins
Cell Differentiation
Mesenchymal Stem Cells
Cell Biology
Original Articles
Cell sorting
Cell biology
Transplantation
SF1
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
biology.protein
Molecular Medicine
Original Article
Stem cell
transplantation
Signal Transduction
Stem Cell Transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 15824934 and 15821838
- Volume :
- 24
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine
- Accession number :
- edsair.doi.dedup.....6b73f802d62047d4a7453d3527c0d78f