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Alpinetin attenuates inflammatory responses by interfering toll-like receptor 4/nuclear factor kappa B signaling pathway in lipopolysaccharide-induced mastitis in mice
- Source :
- International immunopharmacology. 17(1)
- Publication Year :
- 2013
-
Abstract
- Alpinetin, a novel plant flavonoid derived from Alpinia katsumadai Hayata, has been reported to exhibit anti-inflammatory properties. However, the effect of alpinetin on mastitis has not been investigated. The aim of this study was to investigate the protective effect of alpinetin against lipopolysaccharide (LPS)-induced mastitis and to clarify the possible mechanism. In the present study, primary mouse mammary epithelial cells and an LPS-induced mouse mastitis model were used to investigate the effect of alpinetin on mastitis and the possible mechanism. In vivo, we observed that alpinetin significantly attenuated the infiltration of neutrophilic granulocytes, and the activation of myeloperoxidase; down-regulated the level of pro-inflammatory cytokines, including TNF-α, IL-1β and IL-6; inhibited the phosphorylation of IκB-α, NF-κB p65 and the expression of TLR4, caused by LPS. In vitro, we also observed that alpinetin inhibited the expression of TLR4 and the production of TNF-α, IL-1β and IL-6 in LPS-stimulated primary mouse mammary epithelial cells. However, alpinetin could not inhibit the production of IL-1β and IL-6 in TNF-α-stimulated primary mouse mammary epithelial cells. In conclusion, our results suggest that the anti-inflammatory effects of alpinetin against LPS-induced mastitis may be due to its ability to inhibit TLR4-mediated NF-κB signaling pathways. Alpinetin may be a promising potential therapeutic reagent for mastitis treatment.
- Subjects :
- Lipopolysaccharides
Male
Lipopolysaccharide
Immunology
Alpinetin
Mastitis
Biology
Pharmacology
chemistry.chemical_compound
Mice
Mammary Glands, Animal
In vivo
Immunology and Allergy
Animals
Peroxidase
Inflammation
Toll-like receptor
Mice, Inbred BALB C
Dose-Response Relationship, Drug
Molecular Structure
NF-kappa B
Epithelial Cells
In vitro
Toll-Like Receptor 4
chemistry
Gene Expression Regulation
Myeloperoxidase
Flavanones
TLR4
biology.protein
Female
Signal transduction
Subjects
Details
- ISSN :
- 18781705
- Volume :
- 17
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- International immunopharmacology
- Accession number :
- edsair.doi.dedup.....6ba6fd4984c568e758f30266ebf6486b