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Myb-binding protein 1a (Mybbp1a) regulates levels and processing of pre-ribosomal RNA
- Source :
- J. Biol. Chem. 287, 24365-24377 (2012)
- Publication Year :
- 2016
-
Abstract
- Ribosomal RNA gene transcription, co-transcriptional processing, and ribosome biogenesis are highly coordinated processes that are tightly regulated during cell growth. In this study we discovered that Mybbp1a is associated with both the RNA polymerase I complex and the ribosome biogenesis machinery. Using a reporter assay that uncouples transcription and RNA processing, we show that Mybbp1a represses rRNA gene transcription. In addition, overexpression of the protein reduces RNA polymerase I loading on endogenous rRNA genes as revealed by chromatin immunoprecipitation experiments. Accordingly, depletion of Mybbp1a results in an accumulation of the rRNA precursor in vivo but surprisingly also causes growth arrest of the cells. This effect can be explained by the observation that the modulation of Mybbp1a protein levels results in defects in pre-rRNA processing within the cell. Therefore, the protein may play a dual role in the rRNA metabolism, potentially linking and coordinating ribosomal DNA transcription and pre-rRNA processing to allow for the efficient synthesis of ribosomes. Refereed/Peer-reviewed
- Subjects :
- Chromatin Immunoprecipitation
Nucleocytoplasmic Transport Proteins
5.8S ribosomal RNA
Ribosome biogenesis
RNA polymerase II
Biology
Real-Time Polymerase Chain Reaction
Gene Regulation
Nucleolus
Ribosomal RNA (rRNA)
Ribosomal RNA Processing
Transcription Regulation
Biochemistry
Cell Line
Mice
5S ribosomal RNA
RNA Polymerase I
Transcription (biology)
RNA polymerase I
Animals
Humans
RNA Processing, Post-Transcriptional
RNA, Small Interfering
Molecular Biology
General transcription factor
Nuclear Proteins
RNA-Binding Proteins
Cell Biology
Ribosomal RNA
Blotting, Northern
Molecular biology
Myb-binding
Cell biology
DNA-Binding Proteins
Microscopy, Fluorescence
RNA, Ribosomal
biology.protein
Carrier Proteins
ribosomal RNA
Ribosomes
HeLa Cells
Transcription Factors
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- J. Biol. Chem. 287, 24365-24377 (2012)
- Accession number :
- edsair.doi.dedup.....6bc6074d6de8c569e7a2859e7a04fb97