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Adiponectin receptor 1 gene (ADIPOR1) as a candidate for type 2 diabetes and insulin resistance
- Source :
- Diabetes. 53(8)
- Publication Year :
- 2004
-
Abstract
- Considerable data support adiponectin as an important adipose-derived insulin sensitizer that enhances fatty acid oxidation and alters hepatic gluconeogenesis. Adiponectin acts by way of two receptors, ADIPOR1 and ADIPOR2. ADIPOR1 is widely expressed in tissues, including muscle, liver, and pancreas, and binds the globular form of adiponectin with high affinity. To test the hypothesis that sequence variations in or near the ADIPOR1 gene contribute to the risk of developing type 2 diabetes and the metabolic syndrome, we screened the eight exons (including the untranslated exon 1) of the ADIPOR1 gene with flanking intronic sequences and the 5′ and 3′ flanking sequences. We identified 22 single nucleotide polymorphisms (SNPs) in Caucasian and African-American subjects, of which a single nonsynonymous SNP (N44K) in exon 2 was present only in African-American subjects. We typed 14 sequence variants that had minor allele frequencies >5%. No SNP was associated with type 2 diabetes in Caucasians or African Americans, and no SNP was a determinant of insulin sensitivity or insulin secretion among nondiabetic members of high-risk Caucasian families. However, the two alleles of a SNP in the 3′ untranslated region were expressed unequally, and ADIPOR1 mRNA levels were significantly lower among transformed lymphocytes from diabetic African-American individuals than among control cell lines. This altered gene expression might suggest a role for ADIPOR1 in the metabolic syndrome.
- Subjects :
- Untranslated region
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Black People
Single-nucleotide polymorphism
Receptors, Cell Surface
Type 2 diabetes
Biology
White People
Cell Line
Exon
Insulin resistance
Internal medicine
Utah
Internal Medicine
medicine
Humans
Lymphocytes
RNA, Messenger
Promoter Regions, Genetic
Genetics
Adiponectin
Insulin
Chromosome Mapping
Genetic Variation
Exons
medicine.disease
Introns
Europe
Endocrinology
Diabetes Mellitus, Type 2
Metabolic syndrome
Insulin Resistance
Receptors, Adiponectin
Subjects
Details
- ISSN :
- 00121797
- Volume :
- 53
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Diabetes
- Accession number :
- edsair.doi.dedup.....6bca0e83505a80d7b5037881c2d758e0