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Quality control of protein reagents for the improvement of research data reproducibility

Authors :
Bertrand Raynal
Maria Garcia-Alai
Kim Remans
Stefan H. Knauer
Stephan Uebel
Ario de Marco
Mario Lebendiker
Annabel H. A. Parret
André Matagne
Blanca López-Méndez
Nick Berrow
University of Nova Gorica
Barcelona Institute of Science and Technology (BIST)
The Hebrew University of Jerusalem (HUJ)
European Molecular Biology Laboratory [Hamburg] (EMBL)
Universität Bayreuth
Novo Nordisk Foundation Center for Protein Research (CPR)
Faculty of Health and Medical Sciences
University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH)
Université de Liège
European Molecular Biology Laboratory [Heidelberg] (EMBL)
Charles River
Biophysique Moléculaire (Plate-forme)
Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
ARBRE-MOBIEU is supported by European CO-operation in Science and Technology (COST) Action number CA15126.
Source :
Nature Communications, Vol 12, Iss 1, Pp 1-4 (2021), de Marco, A, Berrow, N, Lebendiker, M, Garcia-Alai, M, Knauer, S H, Lopez-Mendez, B, Matagne, A, Parret, A, Remans, K, Uebel, S & Raynal, B 2021, ' Quality control of protein reagents for the improvement of research data reproducibility ', Nature Communications, vol. 12, no. 1, 2795 . https://doi.org/10.1038/s41467-021-23167-z, Nature Communications, Nature Communications, 2021, 12 (1), pp.2795. ⟨10.1038/s41467-021-23167-z⟩
Publication Year :
2021
Publisher :
Nature Portfolio, 2021.

Abstract

Proteins and peptides are amongst the most widely used research reagents but often their quality is inadequate and can result in poor data reproducibility. Here we propose a simple set of guidelines that, when correctly applied to protein reagents should provide more reliable experimental data.

Details

Language :
English
ISSN :
20411723
Volume :
12
Issue :
1
Database :
OpenAIRE
Journal :
Nature Communications
Accession number :
edsair.doi.dedup.....6bcd6239d888cbebe4ed38f254926023
Full Text :
https://doi.org/10.1038/s41467-021-23167-z