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Initial frequency of alleles conferring resistance toBacillus thuringiensispoplar in a field population ofChrysomela tremulae
- Source :
- Proceedings of the Royal Society B: Biological Sciences, Proceedings of the Royal Society B: Biological Sciences, Royal Society, The, 2003, 270 (1517), pp.791-797. ⟨10.1098/rspb.2002.2317⟩, Proceedings of the Royal Society B: Biological Sciences, 2003, 270 (1517), pp.791-797. ⟨10.1098/rspb.2002.2317⟩
- Publication Year :
- 2003
- Publisher :
- The Royal Society, 2003.
-
Abstract
- International audience; Globally, the estimated total area planted with transgenic plants producing Bacillus thuringiensis (Bt) toxins was 12 million hectares in 2001. The risk of target pests becoming resistant to these toxins has led to the implementation of resistance-management strategies. The efficiency and sustainability of these strategies, including the high-dose plus refuge strategy currently recommended for North American maize, depend on the initial frequency of resistance alleles. In this study, we estimated the initial frequencies of alleles conferring resistance to transgenic Bt poplars producing Cry3A in a natural population of the poplar pest Chrysomela tremulae (Coleoptera: Chrysomelidae). We used the F2 screen method developed for detecting resistance alleles in natural pest populations. At least three parents of the 270 lines tested were heterozygous for a major Bt resistance allele. We estimated mean resistance-allele frequency for the period 1999–2001 at 0.0037 (95% confidence interval = 0.000 45–0.0080) with a detection probability of 90%. These results demonstrate that (i) the F2 screen method can be used to detect major alleles conferring resistance to Bt-producing plants in insects and (ii) the initial frequency of alleles conferring resistance to Bt toxin can be close to the highest theoretical values that are expected prior to the use of Bt plants if considering fitness costs and typical mutation rates.
- Subjects :
- Male
0106 biological sciences
Mutation rate
Veterinary medicine
resistance management
[SDV]Life Sciences [q-bio]
Gene Expression
Genetically modified crops
01 natural sciences
Insecticide Resistance
Gene Frequency
Bacillus thuringiensis
General Environmental Science
2. Zero hunger
0303 health sciences
Larva
food and beverages
General Medicine
Plants, Genetically Modified
Coleoptera
Natural population growth
Female
F 2 screen
GENETIQUE DES POPULATIONS
General Agricultural and Biological Sciences
Research Article
Heterozygote
Liriodendron
resistance-allele frequency
Bacterial Toxins
Biology
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
transgenic Bacillus thuringiensis poplar
Botany
Animals
Allele
Pest Control, Biological
Allele frequency
030304 developmental biology
General Immunology and Microbiology
fungi
Chrysomela tremulae
biology.organism_classification
010602 entomology
high-dose plus refuge strategy
PEUPLIER
PEST analysis
RESISTANCE
Subjects
Details
- ISSN :
- 14712954 and 09628452
- Volume :
- 270
- Database :
- OpenAIRE
- Journal :
- Proceedings of the Royal Society of London. Series B: Biological Sciences
- Accession number :
- edsair.doi.dedup.....6bf7ed6def42f2ab5294293ead1c1e82