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In vitro production and cryotolerance of prepubertal and adult goat blastocysts obtained from oocytes collected by laparoscopic oocyte-pick-up (LOPU) after FSH treatment
- Source :
- Reproduction, Fertility and Development. 21:901
- Publication Year :
- 2009
- Publisher :
- CSIRO Publishing, 2009.
-
Abstract
- This study compares the developmental capacity and cryotolerance of embryos produced from oocytes of stimulated prepubertal and adult Sarda goats. Twelve prepubertal and 13 adult goats were each given 110 and 175 IU FSH, respectively, and cumulus–oocyte complexes (COCs) were collected by laparoscopic oocyte-pick-up (LOPU). After in vitro maturation, fertilisation and culture (IVMFC), blastocysts were vitrified, warmed and blastocoel re-expansion and gene expression were evaluated. Prepubertal goats produced a higher COCs number than adults (mean ± s.e.m., 89.67 ± 5.74 and 26.69 ± 3.66, respectively; P < 0.01). Lower developmental competence was demonstrated in the prepubertal oocytes as shown by a higher number of COCs discarded before IVM (21.1% and 14.7% for prepubertals and adults, respectively; P < 0.01) and IVF (23.4% v. 9.1%; P < 0.01) and by the lower cleavage (55.6% and 70.3%, respectively; P < 0.01) and blastocyst rates (24.2% and 33.9%, respectively; P < 0.05). Compared with the adult, prepubertal vitrified/warmed blastocysts showed significantly (P < 0.05) lower in vitro viability, as determined by the re-expansion rate (62.5% and 40.3%). No differences were observed in the time required for blastocoel re-expansion or in cyclin B1, E-cadherin, Na/K ATPase, HSP90β and aquaporin 3 messenger RNA quantity. These results show that in vitro-produced embryos produced from prepubertal goat oocytes have a lower developmental rate and cryotolerance compared with their adult counterparts. However, we can assume that the quality of re-expanded embryos does not differ between the two groups.
- Subjects :
- Ovulation
Time Factors
Cell Survival
Cleavage Stage, Ovum
medicine.medical_treatment
Oocyte Retrieval
Fertilization in Vitro
Reproductive technology
Biology
Oogenesis
Embryo Culture Techniques
Andrology
Endocrinology
Genetics
medicine
Animals
Molecular Biology
Cell Proliferation
Cryopreservation
In vitro fertilisation
Goats
Age Factors
Gene Expression Regulation, Developmental
Embryo culture
Fertility Agents, Female
Anatomy
Oocyte
Cumulus oophorus
In vitro maturation
Blastocyst
medicine.anatomical_structure
Reproductive Medicine
Laparoscopy
Animal Science and Zoology
Folliculogenesis
Follicle Stimulating Hormone
Developmental Biology
Biotechnology
Subjects
Details
- ISSN :
- 10313613
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Reproduction, Fertility and Development
- Accession number :
- edsair.doi.dedup.....6c1c34a3b00345cdfdb8bb2d14a36811
- Full Text :
- https://doi.org/10.1071/rd09015