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Effect of hydrogen sulfide on glycolysis‐based energy production in mouse erythrocytes
- Source :
- Journal of Cellular Physiology. 237:763-773
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Hydrogen sulfide (H2 S) is a gasotransmitter that regulates both physiological and pathophysiological processes in mammalian cells. Recent studies have demonstrated that H2 S promotes aerobic energy production in the mitochondria in response to hypoxia, but its effect on anaerobic energy production has yet to be established. Glycolysis is the anaerobic process by which ATP is produced through the metabolism of glucose. Mammalian red blood cells (RBCs) extrude mitochondria and nucleus during erythropoiesis. These cells would serve as a unique model to observe the effect of H2 S on glycolysis-mediated energy production. The purpose of this study was to determine the effect of H2 S on glycolysis-mediated energy production in mitochondria-free mouse RBCs. Western blot analysis showed that the only H2 S-generating enzyme expressed in mouse RBCs is 3-mercaptopyruvate sulfurtransferase (MST). Supplement of the substrate for MST stimulated, but the inhibition of the same suppressed, the endogenous production of H2 S. Both exogenously administered H2 S salt and MST-derived endogenous H2 S stimulated glycolysis-mediated ATP production. The effect of NaHS on ATP levels was not affected by oxygenation status. On the contrary, hypoxia increased intracellular H2 S levels and MST activity in mouse RBCs. The mitochondria-targeted H2 S donor, AP39, did not affect ATP levels of mouse RBCs. NaHS at low concentrations (3-100 μM) increased ATP levels and decreased cell viability after 3 days of incubation in vitro. Higher NaHS concentrations (300-1000 μM) lowered ATP levels, but prolonged cell viability. H2 S may offer a cytoprotective effect in mammalian RBCs to maintain oxygen-independent energy production.
- Subjects :
- Erythrocytes
Physiology
Clinical Biochemistry
Endogeny
Mitochondrion
7. Clean energy
Mice
03 medical and health sciences
Adenosine Triphosphate
0302 clinical medicine
medicine
Animals
Glycolysis
Hydrogen Sulfide
Viability assay
Hypoxia
030304 developmental biology
Mammals
0303 health sciences
Chemistry
Cell Biology
Metabolism
Cell biology
Red blood cell
medicine.anatomical_structure
030220 oncology & carcinogenesis
Erythropoiesis
Intracellular
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 237
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....6c216eeb9b70d8c656b3ee8f869b2ff0