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CDKN2A gene inactivation in epithelial sporadic ovarian cancer
- Source :
- British Journal of Cancer
- Publication Year :
- 1999
- Publisher :
- Springer Science and Business Media LLC, 1999.
-
Abstract
- The tumour suppressor gene CDKN2A, located on chromosome 9p21, encodes the cell cycle regulatory protein p16. Inactivation of the CDKN2A gene could lead to uncontrolled cell growth. In order to determine the role of CDKN2A in the development of sporadic ovarian cancer, loss of heterozygosity at 9p21–22, homozygous deletion, mutation and methylation status of the CDKN2A gene as well as CDKN2A expression were examined in a panel of serous papillary ovarian cancer. The frequency of loss of heterozygosity (LOH) for one or more informative markers at 9p21–22 was 65% (15/23). The most common deleted region was located between interferon (IFN)-α and D9S171. Homozygous deletions and mutations of the CDKN2A gene were not found. There was no evidence of methylation in exon 1, but methylation in exon 2 of CDKN2A gene was found in 26% (6/23). Absence of CDKN2A gene expression was shown in 27% (6/22) at mRNA level and 21% (4/19) at protein level. These data suggest that the CDKN2A gene is involved in the tumorigenesis of ovarian cancer, but the mechanisms of CDKN2A gene inactivation in serous papillary ovarian cancer remains unclear. © 1999 Cancer Research Campaign
- Subjects :
- Genetic Markers
Cancer Research
Transcription, Genetic
Tumor suppressor gene
RT-PCR
Loss of Heterozygosity
CDKN2A gene
Biology
medicine.disease_cause
Polymerase Chain Reaction
Loss of heterozygosity
Exon
CDKN2A
hemic and lymphatic diseases
9p21–22
medicine
Humans
LOH
RNA, Messenger
CDKN2A Gene Inactivation
neoplasms
Cyclin-Dependent Kinase Inhibitor p16
DNA Primers
Ovarian Neoplasms
Regulation of gene expression
Genes, p16
Homozygote
Chromosome Mapping
Regular Article
DNA Methylation
Cystadenoma, Papillary
Molecular biology
digestive system diseases
stomatognathic diseases
ovarian cancer
Oncology
Protein Biosynthesis
immunohistochemistry
DNA methylation
Cancer research
Female
Chromosomes, Human, Pair 9
Carcinogenesis
Gene Deletion
Subjects
Details
- ISSN :
- 15321827 and 00070920
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- British Journal of Cancer
- Accession number :
- edsair.doi.dedup.....6c283125c0e21de1810abdaa985bbd0d