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Screening of differently expressed genes in human prostate cancer cell lines with different metastasis potentials
- Source :
- Journal of Huazhong University of Science and Technology. 27:582-585
- Publication Year :
- 2007
- Publisher :
- Springer Science and Business Media LLC, 2007.
-
Abstract
- In order to screen the genes differentially expressed in two human prostate cancer cells with different metastasis potentials, suppression subtractive hybridization (SSH) was done twice on human prostate cancer cell line with high potential of metastasis PC3M-1E8 and its synogenetic cell line PC3M-2B4 with low metastasis potential. In the first subtraction PC3M-2B4 was used as tester and PC3M-1E8 as driver and the forward subtractive library was constructed. In the second on the tester and driver were interchanged and the reverse subtractive library was constructed. The screened clones of both libraries were sequenced and Gene Bank homology search was performed. Some clones were confirmed by quantitative real-time PCR. The results showed that two subtractive libraries containing 238 positive clones were constructed. Analysis of 16 sequenced clones randomly picked from two libraries showed that 4 differentially expressed gene fragments were identified as new EST with unknown functions. It was concluded that two subtractive libraries of human prostate cancer cell lines with different metastasis potentials were constructed successfully.
- Subjects :
- Male
Biomedical Engineering
Biology
Biochemistry
Metastasis
Biomaterials
Gene bank
Cell Line, Tumor
Genetics
medicine
Humans
Genomic library
Neoplasm Metastasis
Gene
Gene Library
Earth-Surface Processes
Gene Expression Profiling
Nucleic Acid Hybridization
Prostatic Neoplasms
medicine.disease
Molecular biology
Gene Expression Regulation, Neoplastic
Gene expression profiling
Suppression subtractive hybridization
Cancer cell
Prostate neoplasm
human activities
Subjects
Details
- ISSN :
- 19931352 and 16720733
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Journal of Huazhong University of Science and Technology
- Accession number :
- edsair.doi.dedup.....6c56b32bea5145247e8da8bd7260e8d0