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Faecal microbiota transplant from aged donor mice affects spatial learning and memory via modulating hippocampal synaptic plasticity- And neurotransmission-related proteins in young recipients
- Source :
- Microbiome, Vol 8, Iss 1, Pp 1-19 (2020), Microbiome
- Publication Year :
- 2020
-
Abstract
- Background The gut-brain axis and the intestinal microbiota are emerging as key players in health and disease. Shifts in intestinal microbiota composition affect a variety of systems; however, evidence of their direct impact on cognitive functions is still lacking. We tested whether faecal microbiota transplant (FMT) from aged donor mice into young adult recipients altered the hippocampus, an area of the central nervous system (CNS) known to be affected by the ageing process and related functions. Results Young adult mice were transplanted with the microbiota from either aged or age-matched donor mice. Following transplantation, characterization of the microbiotas and metabolomics profiles along with a battery of cognitive and behavioural tests were performed. Label-free quantitative proteomics was employed to monitor protein expression in the hippocampus of the recipients. We report that FMT from aged donors led to impaired spatial learning and memory in young adult recipients, whereas anxiety, explorative behaviour and locomotor activity remained unaffected. This was paralleled by altered expression of proteins involved in synaptic plasticity and neurotransmission in the hippocampus. Also, a strong reduction of bacteria associated with short-chain fatty acids (SCFAs) production (Lachnospiraceae, Faecalibaculum, and Ruminococcaceae) and disorders of the CNS (Prevotellaceae and Ruminococcaceae) was observed. Finally, the detrimental effect of FMT from aged donors on the CNS was confirmed by the observation that microglia cells of the hippocampus fimbria, acquired an ageing-like phenotype; on the contrary, gut permeability and levels of systemic and local (hippocampus) cytokines were not affected. Conclusion These results demonstrate that age-associated shifts of the microbiota have an impact on protein expression and key functions of the CNS. Furthermore, these results highlight the paramount importance of the gut-brain axis in ageing and provide a strong rationale to devise therapies aiming to restore a young-like microbiota to improve cognitive functions and the declining quality of life in the elderly.
- Subjects :
- Male
Aging
DIVERSITY
Hippocampus
MICROFLORA
Synaptic Transmission
DISEASE
ageing, microbiota, gut-brain axis, faecal microbiota transplant
Mice
0302 clinical medicine
1108 Medical Microbiology
BRAIN
Microbiota ageing, gut, gut-brain axis
0303 health sciences
Neuronal Plasticity
hyppocampus
Microglia
gut-brain axis
GUT MICROBIOTA
SEROTONIN
IMPAIRMENT
Fecal Microbiota Transplantation
Gut microbiome, microbial transplant, ageing, hyppocampus, gut-brain axis
medicine.anatomical_structure
lcsh:QR100-130
Life Sciences & Biomedicine
0605 Microbiology
Microbiology (medical)
EXPRESSION
microbial transplant
Gut–brain axis
Central nervous system
Spatial Learning
Neurotransmission
Biology
Microbiology
lcsh:Microbial ecology
MICROGLIA
03 medical and health sciences
Memory
Neuroplasticity
medicine
Animals
030304 developmental biology
Gut microbiome
Science & Technology
0602 Ecology
Research
Lachnospiraceae
RECOGNITION
Mice, Inbred C57BL
Transplantation
ageing
Ageing
Immunology
Synaptic plasticity
Quality of Life
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20492618
- Database :
- OpenAIRE
- Journal :
- Microbiome, Vol 8, Iss 1, Pp 1-19 (2020), Microbiome
- Accession number :
- edsair.doi.dedup.....6c6571e12ecd0e3444b419241a1d16e9