Additional file 2: of Immune microenvironment modulation unmasks therapeutic benefit of radiotherapy and checkpoint inhibition

Figure S2. Individual tumor growth curves for singlet and dual treatments and CTX/L-NIL gene expression. Subcutaneous established mEER tumors (day 17–18 post tumor cell injection) were treated with individual or dual treatment combinations of PD-1/CTLA-4, CTX/L-NIL, and radiation (RT) according to the same schedule shown in Figs. 1c and 2b. (A) Individual mEER tumor growth curves for 2 experiments, one of which was used for in Fig. 1d (N = 2; n = 7–17 per group). (B) Individual tumor growth curves for singlet and dual treatment combinations of CPR regimen (N = 2–3; n = 12–19). (C) Differential gene expression of CTX/L-NIL treated tumors compared to control tumors compared after 1 week (day 23) of treatment with PD-L1 and PD-L2 noted in red dots (N = 1; n = 9 per group). Blue lines indicate gene 2-fold change point (vertical) and corrected p-value less than 0.0001 (horizontal). (PDF 3329 kb)