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Local delivery of siRNA-loaded calcium phosphate nanoparticles abates pulmonary inflammation
- Source :
- Nanomedicine
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- The local interference of cytokine signaling mediated by siRNA-loaded nanoparticles might be a promising new therapeutic approach to dampen inflammation during pulmonary diseases. For the local therapeutic treatment of pulmonary inflammation, we produced multi-shell nanoparticles consisting of a calcium phosphate core, coated with siRNAs directed against pro-inflammatory mediators, encapsulated into poly(lactic-co-glycolic acid), and coated with a final outer layer of polyethylenimine. Nasal instillation of nanoparticles loaded with a mixture of siRNAs directed against different cytokines to mice suffering from TH1 cell-mediated lung inflammation, or of siRNA directed against NS-1 in an influenza infection model led to a significant reduction of target gene expression which was accompanied by distinct amelioration of lung inflammation in both models. Thus, this study provides strong evidence that the specific and local modulation of the inflammatory response by CaP/PLGA nanoparticle-mediated siRNA delivery could be a promising approach for the treatment of inflammatory disorders of the lung.<br />Graphical Abstract Calcium phosphate nanoparticles functionalized with siRNA were used to treat pulmonary inflammatory diseases. Inhalation of these nanoparticles leads to reduced target gene expression and results in a decreased pathology of the lung.Image 1
- Subjects :
- Calcium Phosphates
0301 basic medicine
Small interfering RNA
medicine.medical_treatment
Pharmaceutical Science
Medicine (miscellaneous)
Nanoparticle
02 engineering and technology
chemistry.chemical_compound
Polylactic Acid-Polyglycolic Acid Copolymer
Polyethyleneimine
General Materials Science
RNA, Small Interfering
Cells, Cultured
Mice, Inbred BALB C
021001 nanoscience & nanotechnology
PLGA
medicine.anatomical_structure
Cytokine
Cytokines
Molecular Medicine
medicine.symptom
0210 nano-technology
Delivery
Materials science
Chemie
Biomedical Engineering
chemistry.chemical_element
Bioengineering
Inflammation
Calcium
Article
03 medical and health sciences
medicine
Animals
Humans
Lactic Acid
Polyethylenimine
Lung
Pneumonia
Influenza
RNAi Therapeutics
030104 developmental biology
chemistry
siRNA
Immunology
Pulmonary inflammation
Cancer research
Nanoparticles
Polyglycolic Acid
Subjects
Details
- ISSN :
- 15499634
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Nanomedicine: Nanotechnology, Biology and Medicine
- Accession number :
- edsair.doi.dedup.....6c891ab90599bd5f4d21e254f2b6cb1b