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Impact of a Multistrain Probiotic Formulation with High Bifidobacterial Content on the Fecal Bacterial Community and Short-Chain Fatty Acid Levels of Healthy Adults
- Source :
- Microorganisms, Vol 8, Iss 492, p 492 (2020), Microorganisms, Volume 8, Issue 4
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- The consumption of probiotic products is continually increasing, supported by growing scientific evidence of their efficacy. Considering that probiotics may primarily affect health (either positively or negatively) through gut microbiota modulation, the first aspect that should be evaluated is their impact on the intestinal microbial ecosystem. In this study, we longitudinally analyzed the bacterial taxonomic composition and organic acid levels in four fecal samples collected over the course of four weeks from 19 healthy adults who ingested one capsule a day for two weeks of a formulation containing at least 70 billion colony-forming units, consisting of 25% lactobacilli and 75% Bifidobacterium animalis subsp. lactis. We found that 16S rRNA gene profiling showed that probiotic intake only induced an increase in a single operational taxonomic unit ascribed to B. animalis, plausibly corresponding to the ingested bifidobacterial strain. Furthermore, liquid chromatography/mass spectrometry revealed a significant increase in the lactate and acetate/butyrate ratio and a trend toward a decrease in succinate following probiotic administration. The presented results indicate that the investigated probiotic formulation did not alter the intestinal bacterial ecosystem of healthy adults and suggest its potential ability to promote colonization resistance in the gut through a transient increase in fecal bifidobacteria, lactic acid, and the acetate/butyrate ratio.
- Subjects :
- Microbiology (medical)
intervention trial
short-chain fatty acids
DESeq2
Butyrate
Colonisation resistance
Gut flora
Microbiology
Article
law.invention
chemistry.chemical_compound
Probiotic
law
Virology
Food science
lcsh:QH301-705.5
Feces
lactate
fecal microbiota
biology
Short-chain fatty acid
biology.organism_classification
succinate
butyrate
Bifidobacterium animalis
Lactic acid
chemistry
lcsh:Biology (General)
acetate
ALDEx2
Subjects
Details
- Language :
- English
- ISSN :
- 20762607
- Volume :
- 8
- Issue :
- 492
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....6c9343984f473b72fba8fd6ec4652065