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Protein kinase WNK3 regulates the neuronal splicing factor Fox-1
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 109(42)
- Publication Year :
- 2012
-
Abstract
- We report an action of the protein kinase WNK3 on the neuronal mRNA splicing factor Fox-1. Fox-1 splices mRNAs encoding proteins important in synaptic transmission and membrane excitation. WNK3, implicated in the control of neuronal excitability through actions on ion transport, binds Fox-1 and inhibits its splicing activity in a kinase activity-dependent manner. Phosphorylation of Fox-1 by WNK3 does not change its RNA binding capacity; instead, WNK3 increases the cytoplasmic localization of Fox-1, thereby suppressing Fox-1–dependent splicing. These findings demonstrate a role of WNK3 in RNA processing. Considering the implication of WNK3 and Fox-1 in disorders of neuronal development such as autism, WNK3 may offer a target for treatment of Fox-1–induced disease.
- Subjects :
- RNA Splicing Factors
Immunoprecipitation
animal diseases
RNA-binding protein
Electrophoretic Mobility Shift Assay
Biology
Protein Serine-Threonine Kinases
Splicing factor
Mice
Two-Hybrid System Techniques
parasitic diseases
Animals
Humans
Protein kinase A
DNA Primers
Gene Library
Multidisciplinary
Reverse Transcriptase Polymerase Chain Reaction
RNA
virus diseases
food and beverages
Brain
RNA-Binding Proteins
Biological Sciences
Deuterium
Cell biology
HEK293 Cells
Biochemistry
RNA splicing
Phosphorylation
population characteristics
Phosphorus Radioisotopes
Subjects
Details
- ISSN :
- 10916490
- Volume :
- 109
- Issue :
- 42
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....6c989d23babecb0acc824a5db93fe9fe