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Arsenic Methylation in Arabidopsis thaliana Expressing an Algal Arsenite Methyltransferase Gene Increases Arsenic Phytotoxicity
- Source :
- Journal of Agricultural and Food Chemistry. 64:2674-2681
- Publication Year :
- 2016
- Publisher :
- American Chemical Society (ACS), 2016.
-
Abstract
- Arsenic (As) contamination in soil can lead to elevated transfer of As to the food chain. One potential mitigation strategy is to genetically engineer plants to enable them to transform inorganic As to methylated and volatile As species. In this study, we genetically engineered two ecotypes of Arabidopsis thaliana with the arsenite (As(III)) S-adenosylmethyltransferase (arsM) gene from the eukaryotic alga Chlamydomonas reinhardtii. The transgenic A. thaliana plants gained a strong ability to methylate As, converting most of the inorganic As into dimethylarsenate [DMA(V)] in the shoots. Small amounts of volatile As were detected from the transgenic plants. However, the transgenic plants became more sensitive to As(III) in the medium, suggesting that DMA(V) is more phytotoxic than inorganic As. The study demonstrates a negative consequence of engineered As methylation in plants and points to a need for arsM genes with a strong ability to methylate As to volatile species.
- Subjects :
- 0106 biological sciences
Arsenites
Transgene
Arabidopsis
chemistry.chemical_element
Chlamydomonas reinhardtii
Genetically modified crops
010501 environmental sciences
Methylation
Plant Roots
Polymerase Chain Reaction
01 natural sciences
Article
Arsenic
chemistry.chemical_compound
Botany
Cacodylic Acid
Soil Pollutants
Arabidopsis thaliana
Arsenite methyltransferase
0105 earth and related environmental sciences
Arsenite
biology
Algal Proteins
fungi
food and beverages
Methyltransferases
General Chemistry
Plants, Genetically Modified
biology.organism_classification
Biodegradation, Environmental
chemistry
Genes, Bacterial
General Agricultural and Biological Sciences
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....6ca5479d857ca4782f63cf06a2b39194