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Polyherbal combination for wound healing: Matricaria chamomilla L. and Punica granatum L
- Source :
- Daru
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- BACKGROUND: Punica granatum L. (pomegranate) with astringent activities and Matricaria chamomilla L. (chamomile) with anti-inflammatory and antioxidant properties are natural remedies used for various skin disorders, including wound healing. OBJECTIVES: This study was conducted to evaluate the individual and combined wound healing activity of the methanol extracts of pomegranate and chamomile flowers. METHODS: After preparing the menthol fraction of pomegranate and chamomile flowers, the content of total phenols, total tannins, and total flavonoids of fractions was measured. For standardization of pomegranate and chamomile fractions, Gallic acid and apigenin-7-O-glucoside contents of them were determined using high-performance liquid chromatography (HPLC). Moreover, their antioxidant activities were examined using DPPH and FRAP tests. The antimicrobial assay was performed against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. Three different concentrations of methanol fraction of each plant and one combination dose of fractions were investigated for their wound healing activities in an excision wound model on the rats’ dorsum. Finally, histopathological studies were done at the end of the experiment. RESULTS: Phytochemical examinations showed high amounts of phenolic compounds in pomegranate flowers, while chamomile flower fractions contained a high amount of total flavonoids. Both fractions, especially pomegranate, had potent antioxidant activity. The best results for wound closure were observed 7 days after wound induction. All treated groups exhibited superior wound contraction compared to their placebo at all measurement times. The combined form of pomegranate and chamomile had better wound healing properties compared to a single therapy, especially on time earlier to wound induction. CONCLUSION: This study represented high antioxidant and wound healing activities for methanol fraction of pomegranate and chamomile flowers, which could be related to their high content of phytochemicals. In comparison with single herb treatment, the combined form of these two fractions in lower concentrations accelerated wound closure. GRAPHICAL ABSTRACT: [Image: see text]
- Subjects :
- Male
Staphylococcus aureus
Antioxidant
Matricaria
Astringent
DPPH
medicine.medical_treatment
Phytochemicals
Anti-Inflammatory Agents
Flowers
01 natural sciences
Antioxidants
Pomegranate
030207 dermatology & venereal diseases
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Phenols
Picrates
medicine
Animals
Gallic acid
Rats, Wistar
Skin
Flavonoids
Wound Healing
integumentary system
Traditional medicine
biology
Plant Extracts
business.industry
Biphenyl Compounds
Streptococcus
Building and Construction
biology.organism_classification
Anti-Bacterial Agents
0104 chemical sciences
010404 medicinal & biomolecular chemistry
Matricaria chamomilla
chemistry
Phytochemical
Punica
Pseudomonas aeruginosa
business
Wound healing
Tannins
Research Article
Subjects
Details
- ISSN :
- 20082231
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- DARU Journal of Pharmaceutical Sciences
- Accession number :
- edsair.doi.dedup.....6ccfbe374fab05be6acfeebef1102fe8