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Cytotoxic Plant Extracts towards Insect Cells: Bioactivity and Nanoencapsulation Studies for Application as Biopesticides
- Source :
- Molecules, Vol 25, Iss 5855, p 5855 (2020), Molecules, Volume 25, Issue 24, Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- The potential of plant extracts as bioinsecticides has been described as a promising field of agricultural development. In this work, the extracts of Punica granatum (pomegranate), Phytolacca americana (American pokeweed), Glandora prostrata (shrubby gromwell), Ulex europaeus (gorce), Tagetes patula (French marigold), Camellia japonica red (camellia), Ruta graveolens (rue or herb-of-grace) were obtained, purified, and their activity against Spodoptera frugiperda (Sf9) insect cells was investigated. From the pool of over twenty extracts obtained, comprising different polarities and vegetable materials, less polar samples were shown to be more toxic towards the insect cell line Sf9. Among these, a dichloromethane extract of R. graveolens was capable of causing a loss of viability of over 50%, exceeding the effect of the commercial insecticide chlorpyrifos. This extract elicited chromatin condensation and the fragmentation in treated cells. Nanoencapsulation assays of the cytotoxic plant extracts in soybean liposomes and chitosan nanostructures were carried out. The nanosystems exhibited sizes lower or around 200 nm, low polydispersity, and generally high encapsulation efficiencies. Release assays showed that chitosan nanoemulsions provide a fast and total extract release, while liposome-based systems are suitable for a more delayed release. These results represent a proof-of-concept for the future development of bioinsecticide nanoformulations based on the cytotoxic plant extracts.<br />This research was funded by COMPETE 2020 program, co-financed by the FEDER and the European Union, PTDC/ASP-AGR/30154/2017 (POCI-01-0145-FEDER-030154). Foundation for Science and Technology (FCT, Portugal), and FEDER-COMPETE-QREN-EU funded research centers CQ-UM(UIDB/00686/2020), CF-UM-UP (UIDB/04650/2020) and REQUIMTE (UIDB/50006/2020).
- Subjects :
- Insecticides
biopesticides
Insecta
plant extracts
Ruta graveolens
Pharmaceutical Science
Sf9
02 engineering and technology
Pomegranate
chlorpyrifos
Analytical Chemistry
Chitosan
chemistry.chemical_compound
Drug Discovery
Phytolacca americana
Food science
0303 health sciences
Proteger a vida terrestre
biology
Lithospermum
food and beverages
Camellia
Fabaceae
021001 nanoscience & nanotechnology
Biological Control Agents
Chemistry (miscellaneous)
Punica
Molecular Medicine
0210 nano-technology
Ciências Agrárias::Biotecnologia Agrária e Alimentar
Biotecnologia Agrária e Alimentar [Ciências Agrárias]
Article
nanoencapsulation
Tagetes
lcsh:QD241-441
03 medical and health sciences
lcsh:Organic chemistry
Animals
Pesticides
Physical and Theoretical Chemistry
030304 developmental biology
Ruta
Science & Technology
Plant Extracts
bioinsecticides
Organic Chemistry
fungi
biology.organism_classification
Ulex europaeus
Nanostructures
Plant Leaves
Biopesticide
Camellia japonica
chemistry
bioactivity
Liposomes
Solvents
Soybeans
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 25
- Issue :
- 5855
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....6cdfffdf27c2307f3dbe677783ce332e