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Panax Notoginseng Saponin Attenuates Gastric Mucosal Epithelial Cell Injury Induced by Dual Antiplatelet Drugs through COX and PI3K/Akt/ VEGF-GSK-3β-RhoA Network Pathway
- Source :
- Chinese Journal of Integrative Medicine. 27:819-824
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- To elucidate the underlying mechanism of Panax notoginseng saponin (PNS) on gastric epithelial cell injury and barrier dysfunction induced by dual antiplatelet (DA). Human gastric mucosal epithelial cell (GES-1) was cultured and divided into 4 groups: a control, a DA, a PNS+DA and a LY294002+PNS+DA group. GES-1 apoptosis was detected by flow cytometry, cell permeability were detected using Transwell, level of prostaglandins E2 (PGE2), 6-keto-prostaglandin F1α (6-keto-PGF1α) and vascular endothelial growth factor (VEGF) in supernatant were measured by enzyme linked immunosorbent assay (ELISA), expression of phosphatidylinositide 3-kinase (PI3K), phosphorylated-PI3K (p-PI3K), Akt, phosphorylated-Akt (p-Akt), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), glycogen synthase kinase-3β (GSK-3β) and Ras homolog gene family member A (RhoA) were measured by Western-blot. DA induced apoptosis and hyper-permeability in GES-1, reduced supernatant level of PGE2, 6-keto-PGF1α and VEGF (P
- Subjects :
- Vascular Endothelial Growth Factor A
RHOA
0211 other engineering and technologies
Panax notoginseng
02 engineering and technology
030226 pharmacology & pharmacy
Flow cytometry
Phosphatidylinositol 3-Kinases
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
021105 building & construction
medicine
Humans
Pharmacology (medical)
Protein kinase B
PI3K/AKT/mTOR pathway
Glycogen Synthase Kinase 3 beta
medicine.diagnostic_test
biology
Epithelial Cells
General Medicine
Saponins
biology.organism_classification
Molecular biology
Vascular endothelial growth factor
Complementary and alternative medicine
chemistry
Apoptosis
Cyclooxygenase 1
biology.protein
Cyclooxygenase
rhoA GTP-Binding Protein
Proto-Oncogene Proteins c-akt
Platelet Aggregation Inhibitors
Subjects
Details
- ISSN :
- 19930402 and 16720415
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Chinese Journal of Integrative Medicine
- Accession number :
- edsair.doi.dedup.....6d030fa42fc0a209fcd745031d7387c1