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Lycopene in the prevention of renal cell cancer in the TSC2 mutant Eker rat model

Authors :
Viraj A. Master
Ramzi M. Mohammad
Birdal Bilir
Nurhan Sahin
Wayne Harris
Karin Wertz
Carlos S. Moreno
Cheryl L. Walker
Adeboye O. Osunkoya
Brian Cross
Kazim Sahin
Karina Ciccone
Omer Kucuk
Shadeah Suleiman
Bradley C. Carthon
Source :
Archives of Biochemistry and Biophysics. 572:36-39
Publication Year :
2015
Publisher :
Elsevier BV, 2015.

Abstract

Renal cell carcinoma (RCC) is the most frequent upper urinary tract cancer in humans and accounts for 80–85% of malignant renal tumors. Eker rat represents a unique animal model to study RCC since these rats develop spontaneous renal tumors and leiomyoma, which may be due to tuberous sclerosis 2 (TSC2) mutation resulting in the activation of the mammalian target of rapamycin (mTOR) pathway. This study examines the role of a lycopene-rich diet in the development of RCC in the TSC2 mutant Eker rat model. Ten-week old female Eker rats (n = 90) were assigned in equal numbers to receive 0,100 or 200 mg/kg of lycopene as part of their daily diet. After 18 months the rats were sacrificed and the kidneys were removed. Immunohistochemical staining with antibodies against mTOR, phospho-S6 and EGFR were performed, as well as hematoxylin–eosin staining for histologic examination of the tumors. Tumors were counted and measured in individual kidneys. Presence of tumor decreased from 94% in control animals to 65% in the experimental group, but the difference was not statistically significant (P< 0.12). However, mean numbers of renal carcinomas were statistically significantly decreased in the lycopene-treated rats (P< 0.008) when compared to untreated controls. In the lycopene group, tumor numbers decreased (P< 0.002) and the numbers tended to decrease linearly (P< 0.003) as supplemental lycopene increased from 0 to 200. Control rats fed only basal diet had a greater length of tumors (23.98 mm) than rats fed lycopene supplement groups (12.90 mm and 11.07 mm) (P

Details

ISSN :
00039861
Volume :
572
Database :
OpenAIRE
Journal :
Archives of Biochemistry and Biophysics
Accession number :
edsair.doi.dedup.....6d0d5041b51c0f0c8fbe4e4fae466645
Full Text :
https://doi.org/10.1016/j.abb.2015.01.006