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Prevention and possible mechanism of a purified Laminaria japonica polysaccharide on adriamycin-induced acute kidney injury in mice
- Source :
- International Journal of Biological Macromolecules. 148:591-600
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- The present work aims to investigate the effects and underlying mechanism of a homogeneous Laminaria japonica polysaccharide (LJP61A) on acute kidney injury (AKI) in mice. According to the results of biochemical and pathological analysis, we concluded that LJP61A could protect kidney from the damage of adriamycin in AKI mice. Compared to the model group, the mRNA level of cytokines (TNF-α, IL-1β and MCP-1) and protein level of mesenchymal markers demsin were decrease by the treatment of LJP61A while the protein levels of podocyte structure markers (Nephrin and WT-1) were increased. Moreover, the adriamycin-induced enhancement of phosphor-p65, phosphor-p38, phosphor-ERK1/2 and phosphor-JNK in the kidney of AKI mice were significantly suppressed by LJP61A. Similar variation was observed in the mRNA and protein levels of TGF-β1 and Smad3. These results suggested that LJP61A prevented acute kidney injury possibly via regulating TGF-β1-mediated Smad3, MAPKs and NF-κB signaling pathways.
- Subjects :
- Male
Biopsy
02 engineering and technology
Pharmacology
Kidney Function Tests
Protective Agents
Polysaccharide
Biochemistry
Podocyte
Nephrin
Mice
03 medical and health sciences
Polysaccharides
Structural Biology
medicine
Animals
Molecular Biology
030304 developmental biology
chemistry.chemical_classification
Biological Products
0303 health sciences
Kidney
Messenger RNA
Antibiotics, Antineoplastic
Molecular Structure
biology
urogenital system
Mesenchymal stem cell
Acute kidney injury
General Medicine
Acute Kidney Injury
021001 nanoscience & nanotechnology
medicine.disease
Immunohistochemistry
Disease Models, Animal
medicine.anatomical_structure
chemistry
Doxorubicin
biology.protein
Cytokines
Inflammation Mediators
Laminaria
Signal transduction
0210 nano-technology
Biomarkers
Subjects
Details
- ISSN :
- 01418130
- Volume :
- 148
- Database :
- OpenAIRE
- Journal :
- International Journal of Biological Macromolecules
- Accession number :
- edsair.doi.dedup.....6d49a5487be7118bb3cf9663ef4740f2