Additional file 1 of The genetic basis of adaptation in phenology in an introduced population of Black Cottonwood (Populus trichocarpa, Torr. & Gray)

Additional file1: Table S1: Schematic of crosses and numbers of individuals produced by a cross between the parent individuals. Mothers are on the left (yellow) and fathers on top (blue). The parents in bold and all offspring are present in the Krusenberg field trial. Table S2: Monthly mean temperature and rainfall information for years 2017 and 2018. Table S3: Drawings, pictures and descriptions of the 6 stages of bud burst. The table is reproduced as given to the field scorers with drawing of stage 3 and pictures of stages 5 and 6 missing. Drawings reproduced on permission of the owner Alfas Pliura of Lithuanian Research Centre for Agriculture and Forestry. Table S4: Autumn coloration scoring scheme for 2017-2018. Original 2017 and 2018 describe the original scoring systems used in the corresponding years. converted 2017 shows, which stages the original 2017 scores correspond in the 2018 scoring system. Table S5: Phytotron simulated seasons temperature, light conditions and humidity information. The simulated winter shaded in gray. During late July and early August of 2018, a breakdown of the climate chambers occurred causing the simulated winter (marked with *) to be interrupted by high temperatures before resumption of simulated winter temperatures. Table S6: Sequencing breadth and depth coverage of the 121 sequenced individuals. Values have been rounded to three decimals. Genotyped parent and non-parent non-offspring individuals shaded with gray. Table S7: Heritabilites of chosen traits. Broad sense heritability is calculated using the Cullis method. * evident overfitting. Table S8: GO -term enrichment analysis results. Table S9: Hypergeometric test results. Results are rounded to 3 decimals. Table S10: All candidate genes identified within 10 kbp of significant slopes. Table S11: Shared candidate genes between our results and Evans et al. (2014) and McKown et al. (2014). Figure S1: Pearson’s correlation between BLUP and mean values of A) bud burst initation in year 2017 (BB2-17), B) autumn coloring initiation in year 2018 (CO3-18), C) bud set completion (BS7) and D) diameter at breast height (DBH-17). * p < 2.2-16. Figure S2: Spearman’s correlation matrix and heatmap of all our chosen 23 traits. Figure S3: Logarithmic p-value Manhattan plot. Figure S4: Logarithmic p-value QQ-plot. Figure S5: Level of LD as function of distance. Figure S6: Venn diagram of candidate genes for both year for A) bud burst initiation (BB2), B) bud burs completion (BB4) C) autumn coloring initiation (CO3), D) autumn coloring completion (CO8) E) leaf shed initiation (LS2) and F) leaf shed completion (LS5).