Back to Search
Start Over
Circulating Tumor Cells: A Review of Non-EpCAM-Based Approaches for Cell Enrichment and Isolation
- Source :
- Clinical Chemistry, Clinical Chemistry, American Association for Clinical Chemistry, 2016, 62 (4), pp.571-581. ⟨10.1373/clinchem.2015.249706⟩, Clinical Chemistry, 2016, 62 (4), pp.571-581. ⟨10.1373/clinchem.2015.249706⟩
- Publication Year :
- 2016
- Publisher :
- HAL CCSD, 2016.
-
Abstract
- BACKGROUND Circulating tumor cells (CTCs) are biomarkers for noninvasively measuring the evolution of tumor genotypes during treatment and disease progression. Recent technical progress has made it possible to detect and characterize CTCs at the single-cell level in blood. CONTENT Most current methods are based on epithelial cell adhesion molecule (EpCAM) detection, but numerous studies have demonstrated that EpCAM is not a universal marker for CTC detection because it fails to detect both carcinoma cells that undergo epithelial-mesenchymal transition (EMT) and CTCs of mesenchymal origin. Moreover, EpCAM expression has been found in patients with benign diseases. A large proportion of the current studies and reviews about CTCs describe EpCAM-based methods, but there is evidence that not all tumor cells can be detected using this marker. Here we describe the most recent EpCAM-independent methods for enriching, isolating, and characterizing CTCs on the basis of physical and biological characteristics and point out the main advantages and disadvantages of these methods. SUMMARY CTCs offer an opportunity to obtain key biological information required for the development of personalized medicine. However, there is no universal marker of these cells. To strengthen the clinical utility of CTCs, it is important to improve existing technologies and develop new, non–EpCAM-based systems to enrich and isolate CTCs.
- Subjects :
- 0301 basic medicine
Epithelial-Mesenchymal Transition
Clinical Biochemistry
[SDV.CAN]Life Sciences [q-bio]/Cancer
Cell Separation
Biology
Cell enrichment
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Circulating tumor cell
[SDV.MHEP.PED] Life Sciences [q-bio]/Human health and pathology/Pediatrics
Antigen
Single-cell analysis
[SDV.CAN] Life Sciences [q-bio]/Cancer
Antigens, Neoplasm
Biomarkers, Tumor
Humans
Epithelial–mesenchymal transition
[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
[SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
[SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics
business.industry
Biochemistry (medical)
Mesenchymal stem cell
Epithelial cell adhesion molecule
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
Epithelial Cell Adhesion Molecule
Neoplastic Cells, Circulating
3. Good health
030104 developmental biology
chemistry
[SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
030220 oncology & carcinogenesis
Immunology
Cancer research
Personalized medicine
Single-Cell Analysis
business
Cell Adhesion Molecules
Subjects
Details
- Language :
- English
- ISSN :
- 00099147 and 15308561
- Database :
- OpenAIRE
- Journal :
- Clinical Chemistry, Clinical Chemistry, American Association for Clinical Chemistry, 2016, 62 (4), pp.571-581. ⟨10.1373/clinchem.2015.249706⟩, Clinical Chemistry, 2016, 62 (4), pp.571-581. ⟨10.1373/clinchem.2015.249706⟩
- Accession number :
- edsair.doi.dedup.....6d9a26b68372ba65ab719f28e940c346