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In Activated Murine Mast Cells, NFATc2 Is Critical for the Production of Autocrine IL-3, Thereby Promoting the Expression of IL-9
- Source :
- The Journal of Immunology. 206:67-76
- Publication Year :
- 2021
- Publisher :
- The American Association of Immunologists, 2021.
-
Abstract
- IL-9 has lent its numerical designation to the Th9 subset of CD4+ Th cells, although it is also produced by additional cell types, including mast cells. It is a pleiotropic cytokine involved in allergic reactions, parasitic infections, autoimmune inflammation, and cancer immunity. In this article, we provide evidence that NFATc2 has contradictory functions in the expression of IL-9 in murine Th9 cells and bone marrow–derived mast cells (BMMC). The basis for this is our observation that the production of IL-9 in NFATc2-deficient Th9 cells is increased, whereas it is decreased in BMMC devoid of NFATc2. In addition, NFATc2 deficiency almost completely abrogates the expression of IL-3 in both cell types. However, selectively in BMMC, the production of IL-9 critically depends on autocrine IL-3 acting via the sustained activation of STAT5 on the expression of IL-9. Furthermore, we demonstrate that IL-3 acts independently and synergistically with IL-1β on the production of IL-9. Taken together, we highlight NFATc2-driven production of autocrine IL-3 as a critical and cell type–specific component for IL-9 expression in BMMC.
- Subjects :
- Cell type
NFATC2
medicine.medical_treatment
Immunology
Cell
Autocrine Communication
Mice
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
STAT5 Transcription Factor
medicine
Animals
Immunology and Allergy
Mast Cells
Autocrine signalling
Cells, Cultured
STAT5
Feedback, Physiological
Mice, Knockout
Mice, Inbred BALB C
NFATC Transcription Factors
biology
Chemistry
Interleukin-9
T-Lymphocytes, Helper-Inducer
Up-Regulation
Cell biology
Mice, Inbred C57BL
Cytokine
medicine.anatomical_structure
biology.protein
Interleukin-3
030215 immunology
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 206
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....6da5ffce75c8df574e30ca932727ec4a