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Epigenetic Changes in the Regulation of Nicotiana tabacum Response to Cucumber Mosaic Virus Infection and Symptom Recovery through Single-Base Resolution Methylomes
- Source :
- Viruses, Volume 10, Issue 8, Viruses, Vol 10, Iss 8, p 402 (2018)
- Publication Year :
- 2018
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2018.
-
Abstract
- Plants have evolved multiple mechanisms to respond to viral infection. These responses have been studied in detail at the level of host immune response and antiviral RNA silencing (RNAi). However, the possibility of epigenetic reprogramming has not been thoroughly investigated. Here, we identified the role of DNA methylation during viral infection and performed reduced representation bisulfite sequencing (RRBS) on tissues of Cucumber mosaic virus (CMV)-infected Nicotiana tabacum at various developmental stages. Differential methylated regions are enriched with CHH sequence contexts, 80% of which are located on the gene body to regulate gene expression in a temporal style. The methylated genes depressed by methyltransferase inhibition largely overlapped with methylated genes in response to viral invasion. Activation in the argonaute protein and depression in methyl donor synthase revealed the important role of dynamic methylation changes in modulating viral clearance and resistance signaling. Methylation-expression relationships were found to be required for the immune response and cellular components are necessary for the proper defense response to infection and symptom recovery.
- Subjects :
- 0301 basic medicine
Nicotiana tabacum
lcsh:QR1-502
lcsh:Microbiology
Cucumber mosaic virus
03 medical and health sciences
RNA interference
Virology
Gene silencing
Epigenetics
DNA methylation
biology
demethylation
Argonaute
biology.organism_classification
Cell biology
RNA silencing
030104 developmental biology
Infectious Diseases
RNAi
gene expression
Subjects
Details
- Language :
- English
- ISSN :
- 19994915
- Database :
- OpenAIRE
- Journal :
- Viruses
- Accession number :
- edsair.doi.dedup.....6da812d35d82f672ffeb6491b5956e60
- Full Text :
- https://doi.org/10.3390/v10080402