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A survey strategy for human respiratory syncytial virus detection among haematopoietic stem cell transplant patients: epidemiological and methodological analysis
- Source :
- Memórias do Instituto Oswaldo Cruz, Volume: 108, Issue: 1, Pages: 119-122, Published: FEB 2013, Memórias do Instituto Oswaldo Cruz., Vol 108, Iss 1, Pp 119-122 (2013), Memórias do Instituto Oswaldo Cruz
- Publication Year :
- 2013
- Publisher :
- Instituto Oswaldo Cruz, Ministério da Saúde, 2013.
-
Abstract
- Human respiratory syncytial virus (HRSV) causes severe infections among children and immunocompromised patients. We compared HRSV infections among Haematopoietic Stem Cell Transplant program (HSCT) patients and children using direct immunofluorescence (DFA), point-of-care RSV Bio Easy(r) and a polymerase chain reaction (PCR) assay. Overall, 102 samples from HSCT patients and 128 from children obtained positivity rate of 18.6% and 14.1% respectively. PCR sensitivity was highest mainly on samples collected after five days of symptoms onset. A combination of both DFA and reverse transcriptase-PCR methods for HSCT high-risk patients is the best diagnostic flow for HRSV diagnosis among these patients.
- Subjects :
- Adult
Microbiology (medical)
medicine.medical_specialty
lcsh:Arctic medicine. Tropical medicine
Adolescent
lcsh:RC955-962
medicine.medical_treatment
respiratory syncytial virus
Short Communications
lcsh:QR1-502
Respiratory Syncytial Virus Infections
Hematopoietic stem cell transplantation
lcsh:Microbiology
Virus
law.invention
Young Adult
law
Nasopharynx
Epidemiology
medicine
Humans
human stem cell transplant patients
Young adult
Child
Direct fluorescent antibody
Polymerase chain reaction
Aged
comparison methods
Reverse Transcriptase Polymerase Chain Reaction
business.industry
Hematopoietic Stem Cell Transplantation
Infant, Newborn
Infant
Middle Aged
Virology
Haematopoiesis
Fluorescent Antibody Technique, Direct
Child, Preschool
Hematologic Neoplasms
Respiratory Syncytial Virus, Human
Immunology
RNA, Viral
Stem cell
business
Brazil
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Memórias do Instituto Oswaldo Cruz, Volume: 108, Issue: 1, Pages: 119-122, Published: FEB 2013, Memórias do Instituto Oswaldo Cruz., Vol 108, Iss 1, Pp 119-122 (2013), Memórias do Instituto Oswaldo Cruz
- Accession number :
- edsair.doi.dedup.....6de3b63ee25cd1fa86e592c5ac84c55a