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Myeloid zinc finger 1 (MZF1) is the most important transcriptional factor for porcine follistatin promoter
- Source :
- Journal of Integrative Agriculture, Vol 14, Iss 7, Pp 1383-1389 (2015)
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Follistatin (FS) is a secreted protein, which was originally isolated from porcine follicular fluid. Expression of follistatin is tightly regulated during porcine growth and development. To study the essential transcriptional regions of the porcine FS promoter, ten primer pairs were designed to amplify segments with different lengths of the FS promoter from −1 800 to +16 bp. The products were then inserted into the pGL3-basic vector to analyze the relative luciferase activity. The results showed that the most remarkable changes of promoter activity were observed between constructs (−302/+16 bp)-FS and (−180/+16 bp)-FS (P
- Subjects :
- promoter
Ecology
biology
Agriculture (General)
Myeloid zinc finger 1
Plant Science
porcine
firefly luciferase
Biochemistry
Follicular fluid
Molecular biology
S1-972
Plasmid
Food Animals
myeloid zinc finger 1
follistatin
biology.protein
Animal Science and Zoology
Luciferase
Vector (molecular biology)
Primer (molecular biology)
Agronomy and Crop Science
Transcription factor
Food Science
Follistatin
Subjects
Details
- ISSN :
- 20953119
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Journal of Integrative Agriculture
- Accession number :
- edsair.doi.dedup.....6df6fbb441248a7b24c01dce5539810f