Back to Search
Start Over
Antifungal Susceptibility, Morphological and Molecular Characterization of Lasiodiplodia theobromae Isolated from a Patient with Keratitis
- Source :
- Mycopathologia. 183(3)
- Publication Year :
- 2017
-
Abstract
- Lasiodiplodia theobromae is a rare ocular pathogen. We report a patient with fungal keratitis caused by L. theobromae. The patient was a 75-year-old male, a farmer with diabetes type II, and no previous history of ocular trauma. Histopathology analysis revealed the presence fungi invading Descemet’s membrane of the cornea. The fungus was characterized by septate, highly bulged fungal filaments involving full corneal thickness in the corresponding histopathology specimens. A dematiaceous mold was isolated and initally identified as L. theobromae by microscopic and macroscopic morphology, and further confirmed by PCR-based determination of internal transcribed spacer (ITS) regions of ribosomal DNA. Antifungal susceptibility tests showed sensitivity to amphotericin B (AMB) and voriconazole ( VRC), and resistance to other azoles, including itraconazole (ITC) and fluconazole (FLC). Corneal transplant was performed. Despite in vitro itraconazole resistance, the patient was successfully treated with oral itraconazole, topical voriconazole and natamycin, combined with ocular injections of amphotericin B and voriconazole.
- Subjects :
- 0301 basic medicine
Male
Microbiological Techniques
Antifungal Agents
Itraconazole
Veterinary (miscellaneous)
030106 microbiology
Microbial Sensitivity Tests
Applied Microbiology and Biotechnology
Microbiology
Polymerase Chain Reaction
Cornea
Corneal Transplantation
03 medical and health sciences
Natamycin
Ascomycota
Drug Resistance, Fungal
Amphotericin B
DNA, Ribosomal Spacer
medicine
Humans
Fungal keratitis
DNA, Fungal
Aged
Voriconazole
Keratitis
biology
business.industry
Histocytochemistry
Sequence Analysis, DNA
biology.organism_classification
medicine.disease
corneal ulcer
eye diseases
Treatment Outcome
Diabetes Mellitus, Type 2
sense organs
business
Agronomy and Crop Science
Eye Infections, Fungal
Fluconazole
medicine.drug
Lasiodiplodia theobromae
Subjects
Details
- ISSN :
- 15730832
- Volume :
- 183
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Mycopathologia
- Accession number :
- edsair.doi.dedup.....6e104da2c242ebf1a23000b45525cc18