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miR-7977 inhibits the Hippo-YAP signaling pathway in bone marrow mesenchymal stromal cells
- Source :
- PLoS ONE, PLoS ONE, Vol 14, Iss 3, p e0213220 (2019)
- Publication Year :
- 2019
- Publisher :
- Public Library of Science, 2019.
-
Abstract
- We and others have demonstrated that various abnormalities of the bone marrow (BM) mesenchymal stromal cells (MSCs) such as aberrant cytokine expression, abnormal hedgehog signaling, and impaired miRNA biogenesis are observed in patients with acute myeloid leukemia (AML). However, underlying mechanisms to induce the dysfunction of BM MSCs have not yet been clarified. We previously showed that AML cells release abundant exosomal miR-7977, which, in turn, enters BM mesenchymal stromal cells (MSCs). However, the precise function of miR-7977 is not known. In this study, we performed transduction of a miR-7977 mimic into MSCs, compared transcriptomes between control-transduced (n = 3) and miR-7977-transduced MSCs (n = 3), and conducted pathway analysis. The array data revealed that the expression of 0.05% of genes was reduced 2-fold and the expression of 0.01% of genes was increased 2-fold. Interestingly, approximately half of these genes possessed a miR-7977 target site, while the other genes did not, suggesting that miR-7977 regulates the gene expression level directly and indirectly. Gene set enrichment analysis showed that the gene sets of Yes-associated protein 1 (YAP1) _up were significantly enriched (p
- Subjects :
- 0301 basic medicine
Apoptosis
Biochemistry
Hematologic Cancers and Related Disorders
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
Small interfering RNAs
Cell Cycle and Cell Division
Cells, Cultured
YAP1
Regulation of gene expression
Multidisciplinary
Stem Cells
Messenger RNA
Cell Cycle
Intracellular Signaling Peptides and Proteins
Hematology
Cell cycle
Myeloid Leukemia
Hedgehog signaling pathway
Cell biology
Nucleic acids
Oncology
Cell Processes
030220 oncology & carcinogenesis
Medicine
Signal transduction
Cellular Types
Research Article
Signal Transduction
Acute Myeloid Leukemia
Science
Biology
Protein Serine-Threonine Kinases
Transfection
Research and Analysis Methods
03 medical and health sciences
microRNA
Leukemias
Genetics
Humans
Hippo Signaling Pathway
Non-coding RNA
Molecular Biology Techniques
Molecular Biology
Adaptor Proteins, Signal Transducing
Cell Proliferation
Reporter gene
Natural antisense transcripts
Mesenchymal stem cell
Biology and Life Sciences
Cancers and Neoplasms
Mesenchymal Stem Cells
YAP-Signaling Proteins
Cell Biology
Phosphoproteins
Gene regulation
MicroRNAs
030104 developmental biology
Gene Expression Regulation
RNA
Gene expression
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....6e535551a1095d0d0e653afc7036e49c