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Interaction of nuclear ERs and GPER in vitellogenesis in zebrafish
- Source :
- The Journal of steroid biochemistry and molecular biology. 189
- Publication Year :
- 2018
-
Abstract
- Estrogens exert their biological functions through the estrogen receptors (ERs). In zebrafish, three nuclear estrogen receptors (nERs) named ERα, ERβ1 and ERβ2 and one membrane-bound G protein-coupled estrogen receptor (GPER) are identified. Vitellogenin (Vtg) is predominantly expressed in liver and strongly response to the stimulation of estrogen. It has been proposed that all three nERs are functionally involved in vitellogenesis and ERα may act as the major mediator in teleost. However, the role of GPER and its interaction with nERs in this process are not yet defined in teleost species. In the present study, we provide genetic evidence for the functional significance of ERα that the expression of Vtg genes (vtg1, vtg2, vtg3) and their response to estradiol stimulation were significantly decreased in esr1 mutant zebrafish. Activation of ERβ1 and ERβ2 induced Vtg expression through ERα. Moreover, the involvement of GPER in vitellogenesis and its interaction with nERs in zebrafish were firstly proposed in this work. Activation of GPER induced Vtg genes expression while inhibition of GPER significantly attenuated the estrogenic effect on Vtg. Both treatments altered the expression levels of nERs, suggesting GPER acts interactively with nERs. Collectively, the involvement of both nERs and GPER in regulation of vitellogenesis is demonstrated. ERα is the central factor, acting interactively with ERβ1, ERβ2 and GPER, and GPER regulates vitellogenesis directly and interactively with nERs.
- Subjects :
- 0301 basic medicine
Male
medicine.drug_class
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
Estrogen receptor
Stimulation
Biochemistry
Receptors, G-Protein-Coupled
03 medical and health sciences
Vitellogenin
0302 clinical medicine
Endocrinology
medicine
Animals
Estrogen Receptor beta
Vitellins
Molecular Biology
Zebrafish
biology
Vitellogenesis
Estrogen Receptor alpha
Cell Biology
Zebrafish Proteins
biology.organism_classification
Cell biology
030104 developmental biology
Estrogen
030220 oncology & carcinogenesis
biology.protein
Molecular Medicine
Estrogen receptor alpha
GPER
Subjects
Details
- ISSN :
- 18791220
- Volume :
- 189
- Database :
- OpenAIRE
- Journal :
- The Journal of steroid biochemistry and molecular biology
- Accession number :
- edsair.doi.dedup.....6e58e9c7ff53712069d08c321a7539b3