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Sema4D expression and secretion are increased by HIF-1α and inhibit osteogenesis in bone metastases of lung cancer
- Source :
- Clinical & Experimental Metastasis. 36:39-56
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Most lung cancer bone metastasis are characterized by osteolytic destruction and osteoblastic activity is significantly decreased, suggesting that hypoxia may play a critical role in the process, but the underlying mechanisms remain unknown. Semaphorin 4D (Sema4D) is a recently discovered osteogenic inhibitory factor that is expressed at high levels in lung cancers. Here, CoCl2-induced hypoxia significantly enhanced the inhibitory effect of lung cancer cell conditioned media on osteoblast differentiation by inducing the expression and secretion of Sema4D in a HIF-1α- but not HIF-2α-dependent manner. Moreover, HIF-1α directly regulated Sema4D expression by binding to bases 1171 to 798 in the Sema4D promoter. Furthermore, hypoxia increased Sema4D secretion by upregulating a disintegrin and metalloproteinase 17 (ADAM17) expression in lung cancer in a HIF-1α-dependent manner. In bone metastasis samples from 49 patients with lung cancer, Sema4D and ADAM17 expression significantly correlated with HIF-1α expression and strongly correlated with a poor differentiation status and osteolytic bone destruction. These results provide the first evidence that HIF-1α-induced Sema4D expression and secretion play important roles in lung cancer osteolytic bone metastasis by inhibiting osteoblast differentiation, thereby providing potential strategies for the treatment of bone metastasis via targeting osteoblasts.
- Subjects :
- Male
0301 basic medicine
Cancer Research
medicine.medical_specialty
Lung Neoplasms
SEMA4D
Bone Neoplasms
Semaphorins
ADAM17 Protein
Adenocarcinoma
03 medical and health sciences
0302 clinical medicine
Antigens, CD
Osteogenesis
Internal medicine
Basic Helix-Loop-Helix Transcription Factors
Biomarkers, Tumor
Tumor Cells, Cultured
medicine
Humans
Secretion
Lung cancer
Metalloproteinase
Osteoblasts
Hematology
Lung
business.industry
Bone metastasis
Osteoblast
General Medicine
Middle Aged
Hypoxia-Inducible Factor 1, alpha Subunit
Prognosis
medicine.disease
Gene Expression Regulation, Neoplastic
030104 developmental biology
medicine.anatomical_structure
Oncology
Culture Media, Conditioned
030220 oncology & carcinogenesis
Carcinoma, Squamous Cell
Cancer research
Female
business
Follow-Up Studies
Subjects
Details
- ISSN :
- 15737276 and 02620898
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Clinical & Experimental Metastasis
- Accession number :
- edsair.doi.dedup.....6e5aaa6fc34aa953b51222336582b256
- Full Text :
- https://doi.org/10.1007/s10585-018-9951-5